His-tag (4C2) monoclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
His-tag (4C2) monoclonal antibody Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6×His epitope tag. Immunogen :
Synthetic peptide His-tag. Conjugate :
Unconjugated Modification :
Unmodification
His-tag (4C2) monoclonal antibody Background :
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6×His epitope tag. Immunogen :
Synthetic peptide His-tag. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of His-tag mAb at 1:5000 dillution Lane1:HEK293F whole cell lysate Lane2:HEK293F whole cell lysate,transfected with pcDNA3.4-Flag-N-RBD-His. -
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Immunological response and protection of mice immunized with plasmid encoding T. gondii glycolytic enzyme Malate Dehydrogenase
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Immunological response and protection of mice immunized with plasmid encoding T. gondii glycolytic enzyme Malate Dehydrogenase
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Expressional and functional interactions of two Apis cerana cerana olfactory receptors
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Development of an ELISA for distinguishing convalescent sera with Mycoplasma hyopneumoniae infection from hyperimmune sera responses to bacterin vaccination in pigs
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Expression, purification and characterization of TMCO1 for structural studies
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Supplementation of H7N9 Virus-Like Particle Vaccine With Recombinant Epitope Antigen Confers Full Protection Against Antigenically Divergent H7N9 Virus in Chickens.
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TET-Yeasate: An engineered yeast whole-cell lysate-based approach for high performance tetracycline degradation
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.