Product Name :
SOCS-1 (V98) polyclonal antibody Background :
The suppressor of cytokine signaling (SOCS) family members are negative regulators of cytokine signal transduction that inhibit the Jak/Stat pathway. The SOCS family consists of at least 8 members including the originally identified cytokine-inducible SH2-containing protein (CIS1), as well as SOCS1-7. Each SOCS family member contains a central SH2 domain and a conserved carboxy-terminal motif designated as the SOCS box. These proteins are important regulators of cytokine signaling, proliferation, differentiation, and immune responses. SOCS1 (suppressor of cytokine signaling 1), also known as JAB (Janus Kinase binding protein), SSI-1 (Stat-induced Stat inhibitor-1), and TIP3 (Tec-interacting protein 3), is a cytokine-regulated SOCS family member that directly inhibits Jak family members through interaction within their kinase activation loop. In addition to inhibiting Jak/Stat signaling, SOCS1 can also negatively regulate Toll-like receptors that contribute to innate immunity. The SOCS box of SOCS1 can trigger ubiquitin-mediated degradation of proteins within and outside of the Jak/Stat pathway. The highest expression of SOCS1 is seen in the thymus and spleen and it plays a critical role in T cell activation and lymphocyte differentiation. SOCS1 also functions as a tumor suppressor protein by inhibiting hematopoietic oncogenes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SOCS-1 (V98) polyclonal antibody detects endogenous levels of SOCS-1 protein. Immunogen :
Synthetic peptide, corresponding to Human SOCS-1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of SOCS-1 (V98) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:SHSY5Y whole cell lysate(40ug) Lane3:CT-26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:The Thymus tissue lysate of Rat(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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