Product Name :
JAK1 (phospho-Y1022) polyclonal antibody Background :
JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-JAK1 (Y1022) polyclonal antibody detects endogenous levels of JAK1 protein only when phosphorylated at Tyr1022. Immunogen :
Synthetic phosphopeptide derived from human JAK1 around the phosphorylation site of Tyrosine 1022. Conjugate :
Unconjugated Modification :
Phosphorylation
JAK1 (phospho-Y1022) polyclonal antibody Background :
JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-JAK1 (Y1022) polyclonal antibody detects endogenous levels of JAK1 protein only when phosphorylated at Tyr1022. Immunogen :
Synthetic phosphopeptide derived from human JAK1 around the phosphorylation site of Tyrosine 1022. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of JAK1 (phospho-Y1022) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate(40ug) LaneB:AD293 whole cell lysate(40ug) LaneC:Hela whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of p-JAK1 (Y1022) pAb in paraffin-embedded human breast cancer tissue at 1:50.
Mouse bone marrow-derived mesenchymal stem cells induce macrophage M2 polarization through the nuclear factor-κB and signal transducer and activator of transcription 3 pathways
PMCID: Pubmed No.:24500984
Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling
PMCID: Pubmed No.:26263390
microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
PMCID: Pubmed No.:27086927
microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
PMCID: Pubmed No.:27086927
Mouse bone marrow-derived mesenchymal stem cells induce macrophage M2 polarization through the nuclear factor-κB and signal transducer and activator of transcription 3 pathways
PMCID: Pubmed No.:24500984
Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling
PMCID: Pubmed No.:26263390
Type II cGMP‑dependent protein kinase inhibits EGF‑induced JAK/STAT signaling in gastric cancer cells
PMCID: Pubmed No.:27357186
Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice
PMCID: Pubmed No.:34194525
Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice.
PMCID: Pubmed No.:36059948
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.