JAK1 polyclonal antibody 
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Product Name :
JAK1 polyclonal antibody Background :
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors. Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats, Shc, insulin receptor substrates, and focal adhesion kinase (FAK), typically contain SH2 or other phospho-tyrosine-binding domains. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
JAK1 polyclonal antibody detects endogenous levels of JAK1 protein. Immunogen :
Synthetic peptide, corresponding to Human JAK1. Conjugate :
Unconjugated Modification :
Unmodification
JAK1 polyclonal antibody Background :
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors. Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats, Shc, insulin receptor substrates, and focal adhesion kinase (FAK), typically contain SH2 or other phospho-tyrosine-binding domains. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
JAK1 polyclonal antibody detects endogenous levels of JAK1 protein. Immunogen :
Synthetic peptide, corresponding to Human JAK1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of JAK1 polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:LOVO whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) -
Immunofluorescent (IF) analyzes of JAK1 pAb in Jurkat at 1:50. -
Immunofluorescent (IF) analyzes of JAK1 pAb in Jurkat at 1:50.
Mouse bone marrow-derived mesenchymal stem cells induce macrophage M2 polarization through the nuclear factor-κB and signal transducer and activator of transcription 3 pathways
PMCID: Pubmed No.:24500984
Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling
PMCID: Pubmed No.:26263390
microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
PMCID: Pubmed No.:27086927
microRNA-23a,-27a and-24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia
PMCID: Pubmed No.:27086927
Mouse bone marrow-derived mesenchymal stem cells induce macrophage M2 polarization through the nuclear factor-κB and signal transducer and activator of transcription 3 pathways
PMCID: Pubmed No.:24500984
Neuronal MHC Class I Expression Is Regulated by Activity Driven Calcium Signaling
PMCID: Pubmed No.:26263390
Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice
PMCID: Pubmed No.:34194525
Traditional Chinese medicine CFF-1 exerts a potent anti-tumor immunity to hinder tumor growth and metastasis in prostate cancer through EGFR/JAK1/STAT3 pathway to inhibit PD-1/PD-L1 checkpoint signaling
PMCID: Pubmed No.:35172257
Dapagliflozin protects against nonalcoholic steatohepatitis in db/db mice.
PMCID: Pubmed No.:36059948
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.