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phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody BS65784
  • Primary:Anti-phospho-ERK1+2 at 1:300 dilution;
Product Namephospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody
Catalog No.BS65784
Swiss-ProtP63085
Host Rabbit
ReactivityHuman,Mouse,Rat,Chicken,Dog,Cow,Horse,Rabbit,Guinea Pig,
ApplicationsWB,ICC,
Application_allWB=1:500-2000,ICC=1:100-500
BiowMW42 kD
Alternative NameERK1 + ERK2 (phospho Thr183/Tyr185); phospho-ERK1/MAPK-1/2(Thr183/Tyr185); ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK1; MAPK2; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK01_MOUSE; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44ERK1; p44MAPK; p44MAPK; PRKM 1; PRKM 1; PRKM 2; PRKM 2; PRKM1; PRKM2; Protein kinase mitogen activated 1; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 2; Protein tyrosine kinas.聽聽
Purification&Purityaffinity purified by Protein A
ConjugateUnconjugated
ModificationPhosphorylation
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Size Price
50ul $208
100ul $398
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Product Name :
phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody
Background :
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Product :
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
phospho-ERK1 + 2 (Thr183/Tyr185) Polyclonal Antibody detects endogenous levels of ERK1 + 2 protein only when phosphorylated at Thr183/Tyr185.
Immunogen :
KLH conjugated Synthesised phosphopeptide derived from mouse ERK1 around the phosphorylation site of Thr183/Tyr185:FL(p-T)E(p-Y)V
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Primary:Anti-phospho-ERK1+2 at 1:300 dilution;
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from apical papilla PMCID:    Pubmed No.:27833148 Wogonin reversed resistant human myelogenous leukemia cells via inhibiting Nrf2 signaling by Stat3/NF-κB inactivation PMCID:    Pubmed No.:28150717 Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation PMCID:    Pubmed No.:26477505 The Mammalian Target of Rapamycin and DNA methyltransferase 1 axis mediates vascular endothelial dysfunction in response to disturbed flow PMCID:    Pubmed No.:29118325 IL-6 Inhibits Starvation-induced Autophagy via the STAT3/Bcl-2 Signaling Pathway PMCID:    Pubmed No.:26549519 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed No.:26456500 DGAEE, a newly synthesized derivative of glycyrrhetinic acid, potently attenuates mouse septic shock via its main metabolite DGA in an IL-10-dependent manner PMCID:    Pubmed 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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