Product Name :
HSD3B2 polyclonal antibody Background :
3 beta-hydroxysteroid dehydrogenase (3β-HSD), also known as HSD3B1 or HSDB3, is a bifunctional enzyme that plays a crucial role in the synthesis of all classes of hormonal steroids. Two human 3β-HSD proteins, designated type I (3β-HSD) and type II (3β-HSD2), are expressed by different genes and function in different areas of the body. Localized to the membrane of the endoplasmic reticulum (ER) and expressed in skin and placenta, 3β-HSD is the type I protein that catalyzes the oxidative conversion of ∂5-ene-3-beta-hydroxy steroid, as well as the conversion of various ketosteroids. Defects in the gene encoding 3β-HSD are associated with classic salt wasting, genital ambiguity, hypogonadism, insulin-resistant polycystic ovary syndrome (PCOS) and an increased susceptibility to prostate cancer. Additionally, congenital deficiency of 3β-HSD activity results in a severe depletion of steroid formation which can be lethal in young children. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, Ph7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSD3B2 polyclonal antibody detects endogenous levels of HSD3B2 protein. Immunogen :
Synthetic peptide, corresponding to Human HSD3B2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of HSD3B2 polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate(20ug) Lane2:The testis tissue lysate of Mouse(20ug) Lane3:RAW264.7 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:A2780 whole cell lysate(40ug) Lane6:SK-OVCAR3 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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