Product Name :
PPP2R5D polyclonal antibody Background :
protein phosphatase 2 regulatory subunit B'delta(PPP2R5D) Homo sapiens The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PP2A-B56-δ Polyclonal Antibody detects endogenous levels of PP2A-B56-δ protein. Immunogen :
The antiserum was produced against synthesized peptide derived from human PPP2R5D. AA range:544-593 Conjugate :
Unconjugated Modification :
Unmodification

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  Western Blot analysis of 293T cells using PP2A-B56-δ Polyclonal Antibody diluted at 1：1000
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  Western blot analysis of lysates from Jurkat and HUVEC cells, using PPP2R5D Antibody. The lane on the right is blocked with the synthesized peptide.
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  Western blot analysis of lysates from Jurkat and HUVEC cells, using PPP2R5D Antibody. The lane on the right is blocked with the synthesized peptide.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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