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Ribosomal Protein L7 Polyclonal Antibody BS65344
  • Western Blot analysis of various cells using Ribosomal Protein L7 Polyclonal Antibody diluted at 1:2000
  • Western Blot analysis of customer's(cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000
  • Western Blot analysis of customer's(cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000
Product NameRibosomal Protein L7 Polyclonal Antibody
Catalog No.BS65344
Swiss-ProtP18124
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB,IHC-p,IF(paraffin section),ELISA
Application_allWestern Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
BiowMW~32 kDa
Alternative NameRPL7; 60S ribosomal protein L7
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Ribosomal Protein L7 Polyclonal Antibody
Background :
ribosomal protein L7(RPL7) Homo sapiens Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical
Product :
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
The antiserum was produced against synthesized peptide derived from human RPL7. AA range:199-248
Immunogen :
The antiserum was produced against synthesized peptide derived from human RPL7. AA range:199-248
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western Blot analysis of various cells using Ribosomal Protein L7 Polyclonal Antibody diluted at 1:2000
  • Western Blot analysis of customer's(cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000
  • Western Blot analysis of customer's(cat sample) using Ribosomal Protein L7 Polyclonal Antibody. Antibody was diluted at 1:2000
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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