CASP7 polyclonal antibody 
Datasheet
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Product Name :
CASP7 polyclonal antibody Background :
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP . During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits . Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD . Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CASP7 polyclonal antibody detects endogenous levels of CASP7 protein. Immunogen :
Synthetic peptide, corresponding to Human CASP7. Conjugate :
Unconjugated Modification :
Unmodification
CASP7 polyclonal antibody Background :
Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP . During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits . Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD . Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CASP7 polyclonal antibody detects endogenous levels of CASP7 protein. Immunogen :
Synthetic peptide, corresponding to Human CASP7. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CASP7 polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug)
PEG-liposomal oxaliplatin induces apoptosis in hunman colorectal cancer cells via Fas/FasL and caspase-8.
PMCID: Pubmed No.:21888623
Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells.
PMCID: Pubmed No.:22710431
Airborne fine particulate matter induces multiple cell death pathways in human lung epithelial cells
PMCID: Pubmed No.:24722831
Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis
PMCID: Pubmed No.:26643564
Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis
PMCID: Pubmed No.:26643564
Airborne fine particulate matter induces multiple cell death pathways in human lung epithelial cells
PMCID: Pubmed No.:24722831
Apoptotic and nonapoptotic function of caspase 7 in spermatogenesis
PMCID: Pubmed No.:26643564
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.