Product Name :
SQSTM1 / p62 polyclonal antibody Background :
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SQSTM1 / p62 polyclonal antibody detects endogenous levels of SQSTM1 / p62 protein. Immunogen :
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SQSTM1 / p62 Conjugate :
Unconjugated Modification :
Unmodification
SQSTM1 / p62 polyclonal antibody Background :
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
SQSTM1 / p62 polyclonal antibody detects endogenous levels of SQSTM1 / p62 protein. Immunogen :
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human SQSTM1 / p62 Conjugate :
Unconjugated Modification :
Unmodification
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SQSTM1 / p62 antibody. Western blot was performed from the immunoprecipitate using SQSTM1 / p62 antibody at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody at 1:1000 dilution.
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Western blot analysis of extracts of various cell lines, using SQSTM1 / p62 antibody at 1:1000 dilution.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.