YAP (Q295) polyclonal antibody 
Datasheet
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MSDS
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Product Name :
YAP (Q295) polyclonal antibody Background :
The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
YAP (Q295) polyclonal antibody detects endogenous levels of YAP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 280-330 of Human YAP. Conjugate :
Unconjugated Modification :
Unmodification
YAP (Q295) polyclonal antibody Background :
The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
YAP (Q295) polyclonal antibody detects endogenous levels of YAP protein. Immunogen :
Synthetic peptide, corresponding to amino acids 280-330 of Human YAP. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of YAP (Q295) pAb at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:MEF whole cell lysate(40ug) Lane3:L02 whole cell lysate(40ug) Lane4:PC3 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of YAP (Q295) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
YAP accelerates Aβ25–35-induced apoptosis through upregulation of Bax expression by interaction with p73
PMCID: Pubmed No.:21562856
Inhibition of Aβ25–35-induced cell apoptosis by Low-power-laser-irradiation (LPLI) through promoting Akt-dependent YAP cytoplasmic translocation
PMCID: Pubmed No.:21945154
GRM7 Regulates Embryonic Neurogenesis via CREB and YAP.
PMCID: Pubmed No.:25921811
Phosphatidylcholine could protect the defect of zearalenone exposure on follicular development and oocyte maturation
PMCID: Pubmed No.:30472698
Mesenchymal stem cell-derived exosomal miR-27b-3p alleviates liver fibrosis via downregulating YAP/LOXL2 pathway
PMCID: Pubmed No.:37328872
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.