MDM2 (F414) polyclonal antibody 
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Product Name :
MDM2 (F414) polyclonal antibody Background :
p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MDM2 (F414) polyclonal antibody detects endogenous levels of MDM2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2. Conjugate :
Unconjugated Modification :
Unmodification
MDM2 (F414) polyclonal antibody Background :
p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MDM2 (F414) polyclonal antibody detects endogenous levels of MDM2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of MDM2 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HepG2 whole cell lysate(40ug) Lane5:Pancl whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of MDM2 (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα
PMCID: Pubmed No.:26776160
The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα
PMCID: Pubmed No.:26776160
D-peptide inhibitors of the p53–MDM2 interaction for targeted molecular therapy of malignant neoplasms
PMCID: Pubmed No.:20660730
The oncoprotein p28GANK establishes a positive feedback loop in β-catenin signaling
PMCID: Pubmed No.:21691299
Targeted delivery of a novel palmitylated D-peptide for antiglioblastoma molecular therapy
PMCID: Pubmed No.:22233211
miR-17-5p/20a are important markers for gastric cancer and murine double minute 2 participates in their functional regulation
PMCID: Pubmed No.:23333058
Phosphorylated Hsp27 activates ATM-dependent p53 signaling and mediates the resistance of MCF-7 cells to doxorubicin-induced apoptosis
PMCID: Pubmed No.:23357534
Sulfite exposure-induced hepatocyte death is not associated with alterations in p53 protein expression
PMCID: Pubmed No.:23973939
Heat shock protein gp96 decreases p53 stability by regulating Mdm2 E3 ligase activity in liver cancer
PMCID: Pubmed No.:25637791
Downregulation of FOXP3 inhibits invasion and immune escape in cholangiocarcinoma
PMCID: Pubmed No.:25623530
Protosappanin B protects PC12 cells against oxygen-glucose deprivation-induced neuronal death by maintaining mitochondrial homeostasis via induction of ubiquitin-dependent p53 protein degradation. (Application: Rat IF)
PMCID: Pubmed No.:25657114
Combination of chrysin and cisplatin promotes the apoptosis of Hep G2 cells by up-regulating p53
PMCID: Pubmed No.:25770930
Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner
PMCID: Pubmed No.:28886428
PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway
PMCID: Pubmed No.:36246398
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.