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Myc (K422) polyclonal antibody BS2462
  • Western blot (WB) analysis of MYC pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Myc (K422) pAb in paraffin-embedded liver cancer tissue at 1:100.
Product NameMyc (K422) polyclonal antibody
Catalog No.BS2462
Swiss-ProtP01106
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 55 kDa
Alternative NameMyc proto-oncogene protein; Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64; MYC; BHLHE39
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Myc (K422) polyclonal antibody
Background :
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Myc (K422) polyclonal antibody detects endogenous levels of proto-oncogene c-Myc protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 401-450 of Human Myc.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of MYC pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Myc (K422) pAb in paraffin-embedded liver cancer tissue at 1:100.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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