Histone H3 (Acetyl-K9) polyclonal antibody 
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MSDS
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Product Name :
Histone H3 (Acetyl-K9) polyclonal antibody Background :
Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ac-Histone H3 (K9) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at K9. Immunogen :
Synthetic peptide including acetyl-lysine 9 of Histone H3. Conjugate :
Unconjugated Modification :
Acetylated
Histone H3 (Acetyl-K9) polyclonal antibody Background :
Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ac-Histone H3 (K9) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at K9. Immunogen :
Synthetic peptide including acetyl-lysine 9 of Histone H3. Conjugate :
Unconjugated Modification :
Acetylated
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Western blot (WB) analysis of Histone H3 (Acetyl-K9) pAb at 1:1000 dilution Lane1:3T3-L1 whole cell lysate(30ug) Lane2:C6 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(30ug) Lane4:A549 whole cell lysate(40ug) Lane5:LOVO whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Histone H3 (Acetyl-K9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
The expression patterns and correlations of claudin-6, methy-CpG binding protein 2, DNA methyltransferase 1, histone deacetylase 1, acetyl-histone H3 and acetyl-histone H4 and their clinicopathological significance in breast invasive ductal carcinomas
PMCID: Pubmed No.:22455563
Association of SIRT1 expression with shear stress induced endothelial progenitor cell differentiation
PMCID: Pubmed No.:22740055
Icariin promotes histone acetylation and attenuates post-stroke cognitive impairment in the central cholinergic circuits of mice
PMCID: Pubmed No.:23370322
Enriched environment improves post-stroke cognitive impairment in mice by potential regulation of acetylation homeostasis in cholinergic circuits
PMCID: Pubmed No.:27637156
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.