p53 polyclonal antibody 
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Product Name :
p53 polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Recombinant full length Human p53. Conjugate :
Unconjugated Modification :
Unmodification
p53 polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Recombinant full length Human p53. Conjugate :
Unconjugated Modification :
Unmodification
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WesternBlot (WB) analysis of p53 polyclonal antibody. -
Immunohistochemistry (IHC) analysis of p53 pAb in paraffin-embedded Endometrial cancer tissue. -
Immunohistochemistry (IHC) analysis of p53 pAb in paraffin-embedded Endometrial cancer tissue.
Lentivirus-mediated Sirt1 shRNA and resveratrol independently induce porcine preadipocyte apoptosis by canonical apoptotic pathway
PMCID: Pubmed No.:23065251
MicroRNA miR-491-5p Targeting both TP53 and Bcl-XL Induces Cell Apoptosis in SW1990 Pancreatic Cancer Cells through Mitochondria Mediated Pathway
PMCID: Pubmed No.:23519249
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells.
PMCID: Pubmed No.:26580119
HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation
PMCID: Pubmed No.:27110324
Roles of p53 and ASF1A in the Reprogramming of Sheep Kidney Cells to Pluripotent Cells.
PMCID: Pubmed No.:26580119
HSP27 Alleviates Cardiac Aging in Mice via a Mechanism Involving Antioxidation and Mitophagy Activation
PMCID: Pubmed No.:27110324
Radiosensitization to X-ray radiation by telomerase inhibitor MST-312 in human hepatoma HepG2 cells
PMCID: Pubmed No.:25596016
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes
PMCID: Pubmed No.:26113535
MCM2 is a therapeutic target of lovastatin in human non-small cell lung carcinomas
PMCID: Pubmed No.:25738322
Protosappanin B protects PC12 cells against oxygen–glucose deprivation-induced neuronal death by maintaining mitochondrial homeostasis via induction of ubiquitin-dependent p53 protein degradation
PMCID: Pubmed No.:25657114
Protective Efficacy of Vitamins C and E on p,p′-DDT-Induced Cytotoxicity via the ROS-Mediated Mitochondrial Pathway and NF-κB/FasL Pathway
PMCID: Pubmed No.:25464339
Emodin promotes the arrest of human lymphoma Raji cell proliferation through the UHRF1‑DNMT3A‑∆Np73 pathways
PMCID: Pubmed No.:28901428
RASSF10 suppresses colorectal cancer growth by activating P53 signaling and sensitizes colorectal cancer cell to docetaxel
PMCID: Pubmed No.:25638156
Design, Synthesis, and Biological Evaluation of a Novel Aminothiol Compound as Potential Radioprotector
PMCID: Pubmed No.:34471464
Inhibition of HMGB1 Suppresses Hepatocellular Carcinoma Progression via HIPK2-Mediated Autophagic Degradation of ZEB1
PMCID: Pubmed No.:33747917
Synthesis and radioprotective effects of novel hybrid compounds containing edaravone analogue and 3-n-butylphthalide ring-opening derivatives
PMCID: Pubmed No.:33963805
The protective effect of small peptides from Periplaneta americana on hydrogen peroxide–induced apoptosis of granular cells
PMCID: Pubmed No.:34155600
Protective effect of small peptides from Periplaneta americana on cyclophosphamide-induced premature ovarian failure
PMCID: Pubmed No.:34729871
Rhabdomyolysis-induced acute kidney injury and concomitant apoptosis induction via ROS-mediated ER stress is efficaciously counteracted by epigallocatechin gallate
PMCID: Pubmed No.:36028100
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.