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VEGF-A polyclonal antibody BS6496
  • Western blot (WB) analysis of VEGF-A polyclonal antibody
Product NameVEGF-A polyclonal antibody
Catalog No.BS6496
Swiss-ProtP15692
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IF/ICC IHC-P
Application_all\"WB: 1:500-1:1000 IF/ICC: 1:50-1:200 IHC-P: 1:50-1:200\"
BiowMW~ 39/43 kDa
Alternative NameVascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
VEGF-A polyclonal antibody
Background :
VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
VEGFA polyclonal antibody detects endogenous levels of VEGFA protein.
Immunogen :
Recombinant full length Human VEGF-A.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of VEGF-A polyclonal antibody
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PMCID:    Pubmed No.:24534483 Activation of Mesenchymal Stem Cells by Macrophages Prompts Human Gastric Cancer Growth through NF-κB Pathway PMCID:    Pubmed No.:24824968 Wogonin inhibits LPS-induced tumor angiogenesis via suppressing PI3K/Akt/NF-κB signaling PMCID:    Pubmed No.:24858369 The quantum of initial transformed cells potentially modulates the type of local inflammation mechanism elicited by surrounding normal epithelial tissues and systemic immune pattern for tumor arrest or progression. PMCID:    Pubmed No.:25561977 LL202 inhibits lipopolysaccharide-induced angiogenesis in vivo and in vitro PMCID:    Pubmed No.:19428938 Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice. PMCID:    Pubmed No.:25474224 Repression of PES1 expression inhibits growth of gastric cancer PMCID:    Pubmed No.:26423399 Preventive Effects of the Chinese Herbal Medicine Prescription Tangkuei Decoction for Frigid Extremities on Sciatic Neuropathy in Streptozotocin-Induced Diabetic Rats PMCID:    Pubmed No.:27057201 Bufalin enhances anti-angiogenic effect of sorafenib via AKT/VEGF signaling PMCID:    Pubmed No.:26782953 Nano-microcapsule basic fibroblast growth factor combined with hypoxia-inducible factor-1 improves random skin flap survival in rats PMCID:    Pubmed No.:26707180 Wogonin inhibits multiple myeloma-stimulated angiogenesis via c-Myc/VHL/HIF-1a signaling axis PMCID:    Pubmed No.:26735336 The quantum of initial transformed cells potentially modulates the type of local inflammation mechanism elicited by surrounding normal epithelial tissues and systemic immune pattern for tumor arrest or progression. PMCID:    Pubmed No.:25561977 LL202 inhibits lipopolysaccharide-induced angiogenesis in vivo and in vitro PMCID:    Pubmed No.:19428938 Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice. 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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