Stat3 (phospho-S727) polyclonal antibody 
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MSDS
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Product Name :
Stat3 (phospho-S727) polyclonal antibody Background :
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Stat3 (S727) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Ser727. Immunogen :
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Serine 727. Conjugate :
Unconjugated Modification :
Phosphorylation
Stat3 (phospho-S727) polyclonal antibody Background :
Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of JAK kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-Stat3 (S727) polyclonal antibody detects endogenous levels of Stat3 protein only when phosphorylated at Ser727. Immunogen :
Synthetic phosphopeptide derived from human Stat3 around the phosphorylation site of Serine 727. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of Stat3 (phospho-S727) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40µg) Lane2:A549 whole cell lysate(40µg) Lane3:L02 whole cell lysate(40µg) Lane4:PC12 whole cell lysate(40µg) Lane5:3T3-L1 whole cell lysate(40µg) -
Immunohistochemistry (IHC) analyzes of p-Stat3 (S727) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of p-Stat3 (S727) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Inhibition of IL-17A Suppresses Enhanced-Tumor Growth in Low Dose Pre-Irradiated Tumor Beds
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Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Additive Suppression of LPS-Induced IL-10 and TNF-α by Pre-treatment of Dexamethasone and SB203580 in a Murine Alveolar Macrophage Cell Line (MH-S)
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New Function of the Adaptor Protein SH2B1 in Brain-Derived Neurotrophic Factor-Induced Neurite Outgrowth
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Inhibitory Effects of Isorhamnetin on the Invasion of Human Breast Carcinoma Cells by Downregulating the Expression and Activity of Matrix Metalloproteinase-2/9
PMCID: Pubmed No.:26359917
CCL5 secreted by tumor associated macrophages may be a new target in treatment of gastric cancer
PMCID: Pubmed No.:26796278
SH2B1β interacts with STAT3 and enhances fibroblast growth factor 1-induced gene expression during neuronal differentiation
PMCID: Pubmed No.:24396070
Hydroxy-safflor yellow A inhibits neuroinflammation mediated by Aβ1–42 in BV-2 cells
PMCID: Pubmed No.:24412680
LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
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Diosmin protects against cerebral ischemia/reperfusion injury through activating JAK2/STAT3 signal pathway in mice
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The classic signalling and trans‐signalling of interleukin‐6 are both injurious in podocyte under high glucose exposure
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Intrathecal Delivery of IL-6 Reactivates the Intrinsic Growth Capacity of Pyramidal Cells in the Sensorimotor Cortex after Spinal Cord Injury
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Additive Suppression of LPS-Induced IL-10 and TNF-α by Pre-treatment of Dexamethasone and SB203580 in a Murine Alveolar Macrophage Cell Line (MH-S)
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Type II cGMP‑dependent protein kinase inhibits EGF‑induced JAK/STAT signaling in gastric cancer cells
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.