Product Name :
PKC ζ (phospho-T560) polyclonal antibody Background :
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PKC ζ (T560) polyclonal antibody detects endogenous levels of PKC ζ protein only when phosphorylated at Thr560 Immunogen :
Synthetic phosphopeptide derived from human PKC ζ around the phosphorylation site of Threonine 560. Conjugate :
Unconjugated Modification :
Phosphorylation
PKC ζ (phospho-T560) polyclonal antibody Background :
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PKC ζ (T560) polyclonal antibody detects endogenous levels of PKC ζ protein only when phosphorylated at Thr560 Immunogen :
Synthetic phosphopeptide derived from human PKC ζ around the phosphorylation site of Threonine 560. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-PKC ζ (T560) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Mouse(40ug) Lane2:The Brain tissue lysate of Rat(40ug) Lane3:HEK293T whole cell lysate(20ug) Lane4:PC3 whole cell lysate(20ug)
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Immunohistochemistry (IHC) analyzes of p-PKC ζ (T560) pAb in paraffin-embedded human brain tissue.
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Immunohistochemistry (IHC) analyzes of p-PKC ζ (T560) pAb in paraffin-embedded human brain tissue.
Hypoxic preconditioning induces neuroprotection against transient global ischemia in adult rats via preserving the activity of Na+/K+-ATPase
PMCID: Pubmed No.:21672574
Norisoboldine inhibits the production of interleukin-6 in fibroblast-like synoviocytes from adjuvant arthritis rats through PKC/MAPK/NF-κB-p65/CREB pathways
PMCID: Pubmed No.:22473817
Paeoniflorin Attenuates Lipopolysaccharide-Induced Permeability of Endothelial Cells: Involvements of F-Actin Expression and Phosphorylations of PI3K/Akt and PKC
PMCID: Pubmed No.:23053726
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.