PI3K p85α (phospho-Tyr607) polyclonal antibody 
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Product Name :
PI3K p85α (phospho-Tyr607) polyclonal antibody Background :
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PI3 kinase p85-α(Tyr607) polyclonal antibody detects endogenous levels of PI3-kinase p85-α protein only when phosphorylated at Tyr607. Immunogen :
Synthetic phosphopeptide derived from human PI3K p85-α around the phosphorylation site of Tyrosine 607. Conjugate :
Unconjugated Modification :
Phosphorylation
PI3K p85α (phospho-Tyr607) polyclonal antibody Background :
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p-PI3 kinase p85-α(Tyr607) polyclonal antibody detects endogenous levels of PI3-kinase p85-α protein only when phosphorylated at Tyr607. Immunogen :
Synthetic phosphopeptide derived from human PI3K p85-α around the phosphorylation site of Tyrosine 607. Conjugate :
Unconjugated Modification :
Phosphorylation
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Western blot (WB) analysis of p-PI3K p85-α(Tyr607) pAb at 1:500 dilution Lane1:H1792 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(40ug) Lane4:The Brain tissue lysate of Rat(40ug)
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Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
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Role of PDGFR-β/PI3K/AKT signaling pathway in PDGF-BB induced myocardial fibrosis in rats
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NMMHC IIA inhibition impedes tissue factor expression and venous thrombosis via Akt/GSK3-NF-B signalling pathways in the endothelium
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Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells
PMCID: Pubmed No.:25193113
NMMHC IIA inhibition impedes tissue factor expression and venous thrombosis via Akt/GSK3-NF-B signalling pathways in the endothelium
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Upregulation of estrogen receptor mediates migration, invasion and proliferation of endometrial carcinoma cells by regulating the PI3K/AKT/mTOR pathway
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GPER-Induced ERK Signaling Decreases Cell Viability of Hepatocellular Carcinoma
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Mingmu Xiaomeng Tablets Restore Autophagy and Alleviate Diabetic Retinopathy by Inhibiting PI3K/Akt/mTOR Signaling
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Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice
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PDB-1 from Potentilla discolor Bunge induces apoptosis and autophagy by downregulating the PI3K/Akt/mTOR signaling pathway in A549 cells
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m6A methyltransferase METTL3 promotes retinoblastoma progression via PI3K/AKT/mTOR pathway
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Downregulating Heparanase-Induced Vascular Normalization: A New Approach To Increase the Bioavailability of Chemotherapeutics in Solid Tumors
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Inhibition of Akt/mTOR/p70S6K Signaling Activity With Huangkui Capsule Alleviates the Early Glomerular Pathological Changes in Diabetic Nephropathy
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Combinatorial treatment of Rhizoma Paridis saponins and sorafenib overcomes the intolerance of sorafenib
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Ursolic acid induces apoptosis and anoikis in colorectal carcinoma RKO cells
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Plasminogen activator Inhibitor-2 inhibits pulmonary arterial smooth muscle cell proliferation in pulmonary arterial hypertension via PI3K/Akt and ERK signaling
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Integrated Network Pharmacology Analysis and Pharmacological Evaluation to Explore the Active Components and Mechanism of Abelmoschus manihot (L.) Medik. on Renal Fibrosis
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Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury
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Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells
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Flower extract of Caragana sinica. ameliorates DSS-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model
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Methyl Helicterate Inhibits Hepatic Stellate Cell Activation Through Modulation of Apoptosis and Autophagy
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Neuroprotective effect of Ginsenoside Re against neurotoxin‑induced Parkinson's disease models via induction of Nrf2
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Hesperidin Reversed Long-Term N-methyl-N-nitro-N-Nitroguanidine Exposure Induced EMT and Cell Proliferation by Activating Autophagy in Gastric Tissues of Rats
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Fangchinoline derivatives inhibits PI3K signaling in vitro and in vivo in non-small cell lung cancer
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MiR-200b-5p inhibits tumor progression in salivary adenoid cystic carcinoma via targeting BTBD1
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Oat β-D-glucan ameliorates type II diabetes through TLR4/PI3K/AKT mediated metabolic axis
PMCID: Pubmed No.:37516222
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.