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PI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody AP0152
  • Western blot (WB) analysis of p-PI3K p85-α/p55γ (Tyr467/199) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
Product NamePI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody
Catalog No.AP0152
Swiss-ProtP27986/Q92569
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB
Application_allWB: 1:500~1:1000
BiowMW~ 55, 85 kDa
Alternative NamePhosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK; PIK3R3
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationPhosphorylation
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50ul $168
100ul $298
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Product Name :
PI3K p85α/p55γ (phospho-Y467/Y199) polyclonal antibody
Background :
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
p-PI3K p85α/p55γ (Y467/Y199) polyclonal antibody detects endogenous levels of PI3K p85α only when phosphorylated at Tyr467. This antibody also detects PI3K p55γ when phosphorylated at Tyr199.
Immunogen :
Synthetic phosphopeptide derived from human PI3K p85α/p55γ around the phosphorylation site of Tyrosine 467/199.
Conjugate :
Unconjugated
Modification :
Phosphorylation
  • Western blot (WB) analysis of p-PI3K p85-α/p55γ (Tyr467/199) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice PMCID:    Pubmed No.:21829168 Madecassoside suppresses migration of fibroblasts from keloids: involvement of p38 kinase and PI3K signaling pathways PMCID:    Pubmed No.:22360962 Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways PMCID:    Pubmed No.:23026290 Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells. PMCID:    Pubmed No.:24877714 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 Effect of baicalin-copper on the induction of apoptosis in human hepatoblastoma cancer HepG2 cells PMCID:    Pubmed No.:25694047 Fucoidan Suppresses Hypoxia-Induced Lymphangiogenesis and Lymphatic Metastasis in Mouse Hepatocarcinoma PMCID:    Pubmed No.:26047481 NMMHC IIA inhibition impedes tissue factor expression and venous thrombosis via Akt/GSK3-NF-B signalling pathways in the endothelium PMCID:    Pubmed No.:25881103 Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium PMCID:    Pubmed No.:26604699 Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy PMCID:    Pubmed No.:26607438 Angiogenin interacts with ribonuclease inhibitor regulating PI3K/AKT/mTOR signaling pathway in bladder cancer cells PMCID:    Pubmed No.:25193113 NMMHC IIA inhibition impedes tissue factor expression and venous thrombosis via Akt/GSK3-NF-B signalling pathways in the endothelium PMCID:    Pubmed No.:25881103 Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium PMCID:    Pubmed No.:26604699 Apelin-13 promotes cardiomyocyte hypertrophy via PI3K-Akt-ERK1/2-p70S6K and PI3K-induced autophagy PMCID:    Pubmed No.:26607438 GPER-Induced ERK Signaling Decreases Cell Viability of Hepatocellular Carcinoma PMCID:    Pubmed No.:33767999 Mingmu Xiaomeng Tablets Restore Autophagy and Alleviate Diabetic Retinopathy by Inhibiting PI3K/Akt/mTOR Signaling PMCID:    Pubmed No.:33927618 Alhagi honey polysaccharides attenuate intestinal injury and immune suppression in cyclophosphamide-induced mice PMCID:    Pubmed No.:34128029 Targeting PI3K/Akt/Nrf2 pathway by glabridin alleviates acetaminophen-induced hepatic injury in rats PMCID:    Pubmed No.:33932887 TXNIP positively regulates the autophagy and apoptosis in the rat müller cell of diabetic retinopathy PMCID:    Pubmed No.:33412212 ERα and/or ERβ activation ameliorates cognitive impairment, neurogenesis and apoptosis in type 2 diabetes mellitus mice PMCID:    Pubmed No.:30201537 Downregulating Heparanase-Induced Vascular Normalization: A New Approach To Increase the Bioavailability of Chemotherapeutics in Solid Tumors PMCID:    Pubmed No.:30059227 Akt phosphorylation regulated by IKKε in response to low shear stress leads to endothelial inflammation via activating IRF3 PMCID:    Pubmed No.:33370582 Neuroprotective effects of novel compound Tozan on cognition, neurogenesis and apoptosis in diabetes PMCID:    Pubmed No.:34734023 Combinatorial treatment of Rhizoma Paridis saponins and sorafenib overcomes the intolerance of sorafenib PMCID:    Pubmed No.:29932973 Activation of ERα and/or ERβ ameliorates cognitive impairment and apoptosis in streptozotocin-induced diabetic mice PMCID:    Pubmed No.:30096284 Ursolic acid induces apoptosis and anoikis in colorectal carcinoma RKO cells PMCID:    Pubmed No.:33549076 Integrated Network Pharmacology Analysis and Pharmacological Evaluation to Explore the Active Components and Mechanism of Abelmoschus manihot (L.) Medik. on Renal Fibrosis PMCID:    Pubmed No.:33061308 Apelin-13 inhibits apoptosis and excessive autophagy in cerebral ischemia/reperfusion injury PMCID:    Pubmed No.:33269749 Let-7b regulates the adriamycin resistance of chronic myelogenous leukemia by targeting AURKB in K562/ADM cells PMCID:    Pubmed No.:32856506 Flower extract of Caragana sinica. ameliorates DSS-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model PMCID:    Pubmed No.:34249260 Methyl Helicterate Inhibits Hepatic Stellate Cell Activation Through Modulation of Apoptosis and Autophagy PMCID:    Pubmed No.:30466104 Pentosan polysulfate ameliorates fibrosis and inflammation markers in SV40 MES13 cells by suppressing activation of PI3K/AKT pathway via miR-446a-3p PMCID:    Pubmed No.:35291969 Next-Generation Sequencing of Circular RNAs Reveals the Molecular Mechanisms of Chondrogenic Differentiation in Human Adipose-derived Stem Cells PMCID:    Pubmed No.:35257277 Melatonin enhances spermatogonia activity through promoting KIAA1429-mediated m6A deposition to activate the PI3K/AKT signaling PMCID:    Pubmed No.:35987159 Hesperidin Reversed Long-Term N-methyl-N-nitro-N-Nitroguanidine Exposure Induced EMT and Cell Proliferation by Activating Autophagy in Gastric Tissues of Rats PMCID:    Pubmed No.:36558440 Neuroprotective effects of novel compound FMDB on cognition, neurogenesis and apoptosis in APP/PS1 transgenic mouse model of Alzheimer's disease PMCID:    Pubmed No.:36893915 MiR-200b-5p inhibits tumor progression in salivary adenoid cystic carcinoma via targeting BTBD1 PMCID:    Pubmed No.:37290676 Oat β-D-glucan ameliorates type II diabetes through TLR4/PI3K/AKT mediated metabolic axis PMCID:    Pubmed No.:37516222
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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