Caspase-8 (T341) polyclonal antibody 
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Product Name :
Caspase-8 (T341) polyclonal antibody Background :
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 8 (T341) polyclonal antibody detects endogenous levels of full length caspase-8, the cleaved intermediate p43/p41 and the caspase-8 active fragment p18. Immunogen :
Synthetic peptide, corresponding to amino acids 310-362 of Human Caspase-8. Conjugate :
Unconjugated Modification :
Unmodification
Caspase-8 (T341) polyclonal antibody Background :
Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 8 (T341) polyclonal antibody detects endogenous levels of full length caspase-8, the cleaved intermediate p43/p41 and the caspase-8 active fragment p18. Immunogen :
Synthetic peptide, corresponding to amino acids 310-362 of Human Caspase-8. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Caspase-8 (T341) polyclonal antibody at 1:500 dilution Lane1:sp2/0 cell lysate treated with colchicine(0.2ng/ml,15mins) Lane2:H9C2 cell lysate with colchicine(0.2ng/ml,15mins) -
Western blot (WB) analysis of Caspase 8 (T341) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with Etoposide for 5min whole cell lysate(40ug) Lane3:HEK293T treated with Etoposide for 2h whole cell lysate(40ug) Lane4:HEK293T treated with Etoposide for 24h whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.