Product Name :
Caspase 9 (E119) polyclonal antibody Background :
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 9 (E119) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa/37 kDa). Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase 9. Conjugate :
Unconjugated Modification :
Unmodification
Caspase 9 (E119) polyclonal antibody Background :
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 9 (E119) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa/37 kDa). Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Caspase 9. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Caspase 9 (E119) polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate Lane2:L02 treated with PBS(1×PBS,PH7.4) for 240min whole cell lysate Lane3:L02 treated with PBS(1×PBS,PH7.4) for 120min whole cell lysate Lane4:L02 treated with PBS(1×PBS,PH7.4) for 60min whole cell lysate Lane5:L02 whole cell lysate
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TRAIL and doxorubicin combination enhances anti-glioblastoma effect based on passive tumor targeting of liposomes
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Smac mimetic compound LCL161 sensitizes esophageal carcinoma cells to radiotherapy by inhibiting the expression of inhibitor of apoptosis protein
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Fluoride‐induced apoptosis and expressions of caspase proteins in the kidney of carp (Cyprinus carpio)
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Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
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A novel anti-proliferative role of HMGA2 in induction of apoptosis through Caspase-2 in primary human fibroblast cells
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Multisite mutation of monomer survivin with enhanced effect on apoptosis regulation of breast cancer cells
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PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
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A novel anti-proliferative role of HMGA2 in induction of apoptosis through Caspase-2 in primary human fibroblast cells
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Smac mimetic compound LCL161 sensitizes esophageal carcinoma cells to radiotherapy by inhibiting the expression of inhibitor of apoptosis protein
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(S)-ZJM-289 Preconditioning Induces a Late Phase Protection Against Nervous Injury Induced by Transient Cerebral Ischemia and Oxygen-Glucose Deprivation
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PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
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Fluoride‐induced apoptosis and expressions of caspase proteins in the kidney of carp (Cyprinus carpio)
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Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
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Triethylenetetramine Synergizes with Pharmacologic Ascorbic Acid in Hydrogen Peroxide Mediated Selective Toxicity to Breast Cancer Cell
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High glucose concentration induces endothelial cell proliferation by regulating cyclin‐D2‐related miR‐98
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Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes
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FXR antagonism of NSAIDs contributes to drug-induced liver injury identified by systems pharmacology approach
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A novel anti-proliferative role of HMGA2 in induction of apoptosis through caspase 2 in primary human fibroblast cells
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Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells
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Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs
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MiR-320a effectively suppresses lung adenocarcinoma cell proliferation and metastasis by regulating STAT3 signals
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Tan IIA inhibits H1299 cell viability through the MDM4‑IAP3 signaling pathway
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Bortezomib enhances the radiosensitivity of hypoxic cervical cancer cells by inhibiting HIF-1α expression
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HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways
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Homoharringtonine suppresses imatinib resistance via the Bcl-6/p53 pathway in chronic myeloid leukemia cell lines
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3B, a novel of photosensitizer, exhibited anti-tumor effects via mitochondrial apoptosis pathway in MCF-7 human breast carcinoma cells
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Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis
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Methyl methanesulfonate induces necroptosis in human lung adenoma A549 cells through the PIG-3-reactive oxygen species pathway
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.