Product Name :
LC3 (phospho-Thr50) polyclonal antibody Background :
Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LC3 (phospho-Thr50) polyclonal antibody detects endogenous levels of LC3A and LC3B protein only when phosphorylated at Thr50. This antibody also recognizes LC3C when phosphorylated at the corresponding residues. Immunogen :
Synthetic phosphopeptide derived from human LC3 around the phosphorylation site of Threonine 50. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of LC3 (phospho-Thr50) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 1 hour whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:HEK293T treated with Etoposide(20ng/ml) for 30 minutes whole cell lysate(40ug) Lane5:HEK293T treated with Etoposide(20ng/ml) for 6 hours whole cell lysate(40ug)
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  Western blot (WB) analysis of LC3 (Phospho-Thr50) pAb at 1:500 dilution Lane1:HEK293T transfected with pEGFP-C3-LC3A whole cell lysate(10ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:GFP protein(100ng) Lane4:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(10ug) Lane5:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(10ug) Lane6:HEK293T whole cell lysate(40ug) Lane7:HEK293T transfected with pEGFP-C3-LC3A whole cell lysate(10ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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