Product Name :
Actin-α3/γ2 (E2) polyclonal antibody Background :
All eukaryotic cells express actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. α actin expression is limited to various types of muscle, whereas β and γ are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of actin stress fibers and focal adhesion, Rac regulates actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Actin-α3/γ2 (E2) polyclonal antibody detects endogenous levels of Actin-α3/γ2 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Actin-α3/γ2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Actin-α3/γ2 (E2) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) Lane5:SGC7901 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of Actin-α3/γ2 (E2) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of Actin-α3/γ2 (E2) pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic and nucleus staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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