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Bax (S4) polyclonal antibody BS1030
  • Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Bax (S4) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
  • Immunohistochemistry (IHC) analyzes of Bax (S4) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
Product NameBax (S4) polyclonal antibody
Catalog No.BS1030
Swiss-ProtQ07812
Host Rabbit
ReactivityHuman
ApplicationsWB IHC IP
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200
BiowMW~ 21 kDa
Alternative NameApoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4; BAX; BCL2L4
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
Bax (S4) polyclonal antibody
Background :
The Bcl-2 gene was isolated at the chromosomal breakpoint of t-bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Bcl-2 is localized to outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes. A related protein, designated Bax p21 (for Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Bax (S4) polyclonal antibody detects endogenous levels of Bax protein.
Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human Bax.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Bax (S4) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
  • Immunohistochemistry (IHC) analyzes of Bax (S4) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
  • Immunohistochemistry (IHC) analyzes of Bax (S4) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.
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PMCID:    Pubmed No.:24276240 Neuroprotective Effects of Clostridium butyricum against Vascular Dementia in Mice via Metabolic Butyrate PMCID:    Pubmed No.:26523278 Phloretin induces apoptosis of non-small cell lung carcinoma A549 cells via JNK1/2 and p38 MAPK pathways PMCID:    Pubmed No.:26503828 Alpinetin inhibits lung cancer progression and elevates sensitization drug-resistant lung cancer cells to cis-diammined dichloridoplatium PMCID:    Pubmed No.:26604699 Apelin-13 protects against apoptosis by activating AMP-activated protein kinase pathway in ischemia stroke PMCID:    Pubmed No.:26631263 Dual inhibition of COX-2/5-LOX blocks colon cancer proliferation, migration and invasion in vitro PMCID:    Pubmed No.:26707712 High glucose concentration induces endothelial cell proliferation by regulating cyclin‐D2‐related miR‐98 PMCID:    Pubmed No.:26840039 Oleuropein, a natural extract from plants, offers neuroprotection in focal cerebral ischemia/reperfusion injury in mice PMCID:    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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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