Product Name :
ERK1/2 (L352) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (L352) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 320-370 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodification
ERK1/2 (L352) polyclonal antibody Background :
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif. Phosphorylation at both the Thr and Tyr residues is required for full enzymatic activation. In response to activation, MAP kinases phos-phorylate downstream components on Serine and Threonine. Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1. The ERK family has three additional members: ERK 3, ERK 5 and ERK 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ERK1/2 (L352) polyclonal antibody detects endogenous levels of ERK1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 320-370 of Human ERK1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ERK1/2 (L352) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
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Immunohistochemistry (IHC) analyzes of ERK1/2 (L352) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
PTP1B promotes cell proliferation and metastasis through activating src and ERK1/2 in non-small cell lung cancer
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Anti-invasive effect of gambogic acid in MDA-MB-231 human breast carcinoma cells
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Lipoxin A 4 Inhibits Transition of Epithelial to Mesenchymal Cells in Proximal Tubules
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Endothelial cells dysfunction induced by silica nanoparticles through oxidative stress via JNK/P53 and NF-kB pathways
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Ginsenoside Rg1 protects against hydrogen peroxide-induced cell death in PC12 cells via inhibiting NF-kB activation
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The neuroprotective effects of Lonicera japonica THUNB. against hydrogen peroxide-induced apoptosis via phosphorylation of MAPKs and PI3K/Akt in SH-SY5Y cells
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Loganin protects against hydrogen peroxide-induced apoptosis by inhibiting phosphorylation of JNK, p38, and ERK 1/2 MAPKs in SH-SY5Y cells
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HOXB7 as a prognostic factor and mediator of colorectal cancer progression
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Isomalto oligosaccharide sulfate inhibits tumor growth and metastasis of hepatocellular carcinoma in nude mice
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Hippocampal Neuronal Nitric Oxide Synthase Mediates the Stress-Related Depressive Behaviors of Glucocorticoids by Downregulating Glucocorticoid Receptor
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Monoclonal antibody to six transmembrane epithelial antigen of prostate-4 influences insulin sensitivity by attenuating phosphorylation of P13K (P85) and Akt: Possible mitochondrial mechanism
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Methyl-1-hydroxy-2-naphthoate, a novel naphthol derivative, inhibits lipopolysaccharide-induced inflammatory response in macrophages via suppression of NF-jB, JNK and p38 MAPK pathways
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Transforming growth factor-b1 induces epithelial-tomesenchymal transition in human lung cancer cells via PI3K/Akt and MEK/Erk1/2 signaling pathways
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Maltose-binding protein isolated from Escherichia coli induces Toll-like receptor 2-mediated viability in U937 cells
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XJP-1, a novel ACEI, with anti-inflammatory properties in HUVECs
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Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Injury by Inhibiting ROS-Activated ERK1/2 and p38MAPK Signaling Pathways in PC12 Cells
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MiR-146a suppresses tumor growth and progression by targeting EGFR pathway and in a p-ERK-dependent manner in castration-resistant prostate cancer
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ROCK1 induces ERK nuclear translocation in PDGF-BB-stimulated migration of rat vascular smooth muscle cells
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Insulin-like growth factor 1 enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells via ERK and JNK MAPK pathways
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Oroxylin A inhibits matrix metalloproteinase-2/9 expression and activation by up-regulating tissue inhibitor of metalloproteinase-2 and suppressing the ERK1/2 signaling pathway
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III-10, a newly synthesized flavonoid, induced differentiation of human U937 leukemia cells via PKCδ activation
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Depletion of intracellular zinc induced apoptosis in cultured hippocampal neurons through Raf/MEK/ERK pathways
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E Platinum, a newly synthesized platinum compound, induces autophagy via inhibiting phosphorylation of mTOR in gastric carcinoma BGC-823 cells
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Madecassoside suppresses migration of fibroblasts from keloids: involvement of p38 kinase and PI3K signaling pathways
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Disruption of mindin exacerbates cardiac hypertrophy and fibrosis
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VI-14, a novel flavonoid derivative, inhibits migration and invasion of human breast cancer cells
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Effect of Heparin-Derived Oligosaccharide on Vascular Smooth Muscle Cell Proliferation
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Suppression of endothelial cell adhesion by XJP-1, a new phenolic compound derived from banana peel
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siRNA against plasminogen activator inhibitor-1 ameliorates bleomycin-induced lung fibrosis in rats
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Oroxylin A prevents inflammation-related tumor through down-regulation of inflammatory gene expression by inhibiting NF-κB signaling
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Plasminogen activator inhibitor-1 promotes the proliferation and inhibits the apoptosis of pulmonary fibroblasts by Ca2 + signaling
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Osteogenesis and angiogenesis induced by porous β-CaSiO3/PDLGA composite scaffold via activation of AMPK/ERK1/2 and PI3K/Akt pathways
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1,2,3,4,6-penta-O-galloyl-β-D-glucose protects PC12 Cells from MPP+-mediated cell death by inducing heme oxygenase-1 in an ERK- and Akt-dependent manner
PMCID: Pubmed No.:23073806
Effect of Heparin-Derived Oligosaccharide on Vascular Smooth Muscle Cell Proliferation and the Signal Transduction Mechanisms Involved
PMCID: Pubmed No.:23097139
Toosendanin induces apoptosis through suppression of JNK signaling pathway in HL-60 cells
PMCID: Pubmed No.:23111283
CD133 Affects the Invasive Ability of HCT116 Cells by Regulating TIMP-2
PMCID: Pubmed No.:23195431
MicroRNA-133 inhibits cell proliferation, migration and invasion by targeting epidermal growth factor receptor and its downstream effector proteins in bladder cancer
PMCID: Pubmed No.:23206218
Oroxylin A, a classical natural product, shows a novel inhibitory effect on angiogenesis induced by lipopolysaccharide
PMCID: Pubmed No.:23238475
Dietary flavonoid genistein induces Nrf2 and phase II detoxification gene expression via ERKs and PKC pathways and protects against oxidative stress in Caco-2 cells
PMCID: Pubmed No.:23255485
The viral oncogene Np9 acts as a critical molecular switch for co-activating β-catenin, ERK, Akt and Notch1 and promoting the growth of human leukemia stem/progenitor cells
PMCID: Pubmed No.:23307033
Rho-associated protein kinase isoforms stimulate proliferation of vascular smooth muscle cells through ERK and induction of cyclin D1 and PCNA
PMCID: Pubmed No.:23402758
Inhibitory effect of oleanolic acid on hepatocellular carcinoma via ERK-p53-mediated cell cycle arrest and mitochondrial-dependent apoptosis
PMCID: Pubmed No.:23404993
Holothurian Glycosaminoglycan Inhibits Metastasis and Thrombosis via Targeting of Nuclear Factor-κB/Tissue Factor/Factor Xa Pathway in Melanoma B16F10 Cells
PMCID: Pubmed No.:23437168
Identification of oligomer proanthocyanidins (F2) isolated from grape seeds as a formyl peptide receptor 1 partial agonist
PMCID: Pubmed No.:23523627
PM2.5 induces Nrf2-mediated defense mechanisms against oxidative stress by activating PIK3/AKT signaling pathway in human lung alveolar epithelial A549 cells
PMCID: Pubmed No.:23525690
Suppression of nuclear factor erythroid 2-related factor 2 via extracellular signal-regulated kinase contributes to bleomycin-induced oxidative stress and fibrogenesis
PMCID: Pubmed No.:23570914
miR-145 inhibits isoproterenol-induced cardiomyocyte hypertrophy by targeting the expression and localization of GATA6.
PMCID: Pubmed No.:23624080
4-Hydroxybenzyl Alcohol Confers Neuroprotection Through Up-Regulation of Antioxidant Protein Expression.
PMCID: Pubmed No.:23624876
S-nitrosylation of ERK inhibits ERK phosphorylation and induces apoptosis
PMCID: Pubmed No.:23657295
MiR-339-5p Regulates the Growth, Colony Formation and Metastasis of Colorectal Cancer Cells by Targeting PRL-1
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Uric acid induces oxidative stress and growth inhibition by activating adenosine monophosphate-activated protein kinase and extracellular signal-regulated kinase signal pathways in pancreatic β cells
PMCID: Pubmed No.:23707617
Dihydrotestosterone alters urocortin levels in human umbilical vein endothelial cells.
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An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer
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Cuprous oxide nanoparticles inhibit angiogenesis via down regulation of VEGFR2 expression
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Potential Application of Hydrolyzed Fish Collagen for Inducing the Multidirectional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
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GPER-mediated proliferation and estradiol production in breast cancer-associated fibroblasts.
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ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes
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LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
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Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways
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Effect of Tenuifoliside A isolated from Polygala tenuifolia on the ERK and PI3K pathways in C6 glioma cells.
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The IL-6-STAT3 axis mediates a reciprocal crosstalk between cancer-derived mesenchymal stem cells and neutrophils to synergistically prompt gastric cancer progression.
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Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
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Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB)
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Baicalein suppresses 17-β-estradiol-induced migration, adhesion and invasion of breast cancer cells via the G protein-coupled receptor 30 signaling pathway
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Frequent amplification of PTP1B is associated with poor survival of gastric cancer patients
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PTP1B promotes cell proliferation and metastasis through activating src and ERK1/2 in non-small cell lung cancer
PMCID: Pubmed No.:25617799
Anti-inflammatory potential of β-cryptoxanthin against LPS-induced inflammation in mouse Sertoli cells
PMCID: Pubmed No.:26686910
Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway
PMCID: Pubmed No.:25213258
Arctigenin but not arctiin acts as the major effective constituent of Arctium lappa L. fruit for attenuating colonic inflammatory response induced by dextran sulfate sodium in mice. (Application: Mouse WB)
PMCID: Pubmed No.:25284342
An Apple Oligogalactan Potentiates the Growth Inhibitory Effect of Celecoxib on Colorectal Cancer
PMCID: Pubmed No.:24274457
Anti-inflammatory potential of β-cryptoxanthin against LPS-induced inflammation in mouse Sertoli cells
PMCID: Pubmed No.:26686910
Cuprous oxide nanoparticles inhibit angiogenesis via down regulation of VEGFR2 expression
PMCID: Pubmed No.:24499922
Potential Application of Hydrolyzed Fish Collagen for Inducing the Multidirectional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
PMCID: Pubmed No.:24359018
GPER-mediated proliferation and estradiol production in breast cancer-associated fibroblasts.
PMCID: Pubmed No.:24481325
ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes
PMCID: Pubmed No.:24624962
LFG-500 Inhibits the Invasion of Cancer Cells via Down-Regulation of PI3K/AKT/NF-κB Signaling Pathway
PMCID: Pubmed No.:24618693
Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways
PMCID: Pubmed No.:24682673
provides growth and metastasis advantage for human hepatocellular carcinoma
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Heat shock protein 60 stimulates the migration of vascular smooth muscle cells via Toll-like receptor 4 and ERK MAPK activation
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Antiinflammation Effects and Mechanisms Study of Geniposide on Rats with Collagen‐Induced Arthritis
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Regulation of RAW 264.7 cell-mediated immunity by polysaccharides from Agaricus blazei Murill via the MAPK signal transduction pathway
PMCID: Pubmed No.:28322382
Anti-inflammatory potential of β-cryptoxanthin against LPS-induced inflammation in mouse Sertoli cells
PMCID: Pubmed No.:26686910
Pretreatment with low dose etomidate prevents etomidate-induced rat adrenal insufficiency by regulating oxidative stress-related MAPKs and apoptosis
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Effects of Chronic REM Sleep Restriction on D1 Receptor and Related Signal Pathways in Rat Prefrontal Cortex
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The regulation of SKF38393 on the signaling pathway of dopamine D1 receptor in hippocampus during chronic sleep deprivation
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Increases in the expression of Na+/H+ exchanger 1 and 3 are associated with insulin signalling in the ruminal epithelium
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HCV core protein binds to gC1qR to induce A20 expression and inhibit cytokine production through MAPKs and NF-κB signaling pathways
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Melatonin promotes the proliferation of GC-1 spg cells by inducing metallothionein-2 expression through ERK1/2 signaling pathway activation
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HCV Core Protein Induces Chemokine CCL2 and CXCL10 Expression Through NF-κB Signaling Pathway in Macrophages.
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Loss of FGFR3 Delays Acute Myeloid Leukemogenesis by Programming Weakly Pathogenic CD117-Positive Leukemia Stem-Like Cells.
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Fatty acid oxidation promotes reprogramming by enhancing oxidative phosphorylation and inhibiting protein kinase C
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Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis
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Loss of FGFR3 Accelerates Bone Marrow Suppression-Induced Hematopoietic Stem and Progenitor Cell Expansion by Activating FGFR1-ELK1-Cyclin D1 Signaling
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Quinalizarin, a specific CK2 inhibitor, can reduce icotinib resistance in human lung adenocarcinoma cell lines
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Anti-Cancer Effects of Sulfasalazine and Vitamin E Succinate in MDA-MB 231 Triple-Negative Breast Cancer Cells
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TRIB2 regulates the expression of miR‑33a‑5p through the ERK/c‑Fos pathway to affect the imatinib resistance of chronic myeloid leukemia cells
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NCX1 disturbs calcium homeostasis and promotes RANKL-induced osteoclast differentiation by regulating JNK/c-Fos/NFATc1 signaling pathway in multiple myeloma
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.