Histone H3 (K4) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Histone H3 (K4) polyclonal antibody Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Histone H3 (K4) polyclonal antibody detects endogenous levels of Histone H3 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Histone H3. Conjugate :
Unconjugated Modification :
Unmodification
Histone H3 (K4) polyclonal antibody Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination are used to mark nucleosomes to create chromatin domains with a range of functions. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Histone H3 (K4) polyclonal antibody detects endogenous levels of Histone H3 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminal of Human Histone H3. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Histone H3 (K4) polyclonal antibody at 1:500 dilution Lane1:DLD cell lysate Lane2:Mouse colon tissue lysate Lane3:Mouse heart tissue lysate Lane4:Rat heart tissue lysate -
Immunohistochemistry (IHC) analyzes of Histone H3 (K4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of Histone H3 (K4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
MicroRNA-105 is involved in TNF-α-related tumor microenvironment enhanced colorectal cancer progression
PMCID: Pubmed No.:29238068
Effects of moderate exercise over different phases on age-related physiological dysfunction in testes of SAMP8 mice
PMCID: Pubmed No.:23751407
Activation of Nrf2-ARE signal pathway protects the brain from damage induced by epileptic seizure
PMCID: Pubmed No.:24333359
MTA enhances the odonto/osteogenic capacity of stem cells from inflammatory dental pulps via NF‐κB pathway
PMCID: Pubmed No.:24102926
Mature miR-183, negatively regulated by transcription factor GATA3, promotes 3T3-L1 adipogenesis through inhibition of the canonical Wnt/β-catenin signaling pathway by targeting LRP6.
PMCID: Pubmed No.:24556500
Wogonin inhibits LPS-induced tumor angiogenesis via suppressing PI3K/Akt/NF-κB signaling
PMCID: Pubmed No.:24858369
miR-135a-5p inhibits 3T3-L1 adipogenesis through activation of canonical Wnt/β-catenin signaling
PMCID: Pubmed No.:24850830
Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer
PMCID: Pubmed No.:25546612
Prophylactic lithium alleviates splenectomy-induced cognitive dysfunction possibly by inhibiting hippocampal TLR4 activation in aged rats
PMCID: Pubmed No.:25839444
Antidiabetic drugs restore abnormal transport of amyloid-β across the blood–brain barrier and memory impairment in db/db mice
PMCID: Pubmed No.:26211973
FV‐429 induces apoptosis and inhibits glycolysis by inhibiting Akt‐mediated phosphorylation of hexokinase II in MDA‐MB‐231 cells
PMCID: Pubmed No.:26258875
LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
PMCID: Pubmed No.:26418365
Knockdown of hippocampal cysteinyl leukotriene receptor 1 prevents depressive behavior and neuroinflammation induced by chronic mild stress in mice
PMCID: Pubmed No.:26546369
Epigenetic silencing of miR-490-3p promotes development of an aggressive colorectal cancer phenotype through activation of the wnt/β-catenin signaling pathway
PMCID: Pubmed No.:27037061
Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer
PMCID: Pubmed No.:25546612
LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway
PMCID: Pubmed No.:26418365
Knockdown of hippocampal cysteinyl leukotriene receptor 1 prevents depressive behavior and neuroinflammation induced by chronic mild stress in mice
PMCID: Pubmed No.:26546369
Epigenetic silencing of miR-490-3p promotes development of an aggressive colorectal cancer phenotype through activation of the wnt/β-catenin signaling pathway
PMCID: Pubmed No.:27037061
Activation of Nrf2-ARE signal pathway protects the brain from damage induced by epileptic seizure
PMCID: Pubmed No.:24333359
MTA enhances the odonto/osteogenic capacity of stem cells from inflammatory dental pulps via NF‐κB pathway
PMCID: Pubmed No.:24102926
Mature miR-183, negatively regulated by transcription factor GATA3, promotes 3T3-L1 adipogenesis through inhibition of the canonical Wnt/β-catenin signaling pathway by targeting LRP6.
PMCID: Pubmed No.:24556500
Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.
PMCID: Pubmed No.:33408776
Cloxiquine, a traditional antituberculosis agent, suppresses the growth and metastasis of melanoma cells through activation of PPARγ
PMCID: Pubmed No.:31138783
The novel methyltransferase SETD4 regulates TLR agonist-induced expression of cytokines through methylation of lysine 4 at histone 3 in macrophages
PMCID: Pubmed No.:31376731
Posttranslational S-nitrosylation modification regulates HMGB1 secretion and promotes its proinflammatory and neurodegenerative effects
PMCID: Pubmed No.:36103834
Yin Yang 1-Induced Long Noncoding RNA DUXAP9 Drives the Progression of Oral Squamous Cell Carcinoma by Blocking CDK1-Mediated EZH2 Degradation
PMCID: Pubmed No.:37401236
The kinesin-14 family motor protein KIFC2 promotes prostate cancer progression by regulating p65
PMCID: Pubmed No.:37716704
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.