HSP90α (P717) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
HSP90α (P717) polyclonal antibody Background :
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP90α (P717) polyclonal antibody detects endogenous levels of HSP90α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 671-720 of Human HSP90α. Conjugate :
Unconjugated Modification :
Unmodification
HSP90α (P717) polyclonal antibody Background :
In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
HSP90α (P717) polyclonal antibody detects endogenous levels of HSP90α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 671-720 of Human HSP90α. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of HSP90α (P717) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:L02 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of HSP90α (P717) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of HSP90α (P717) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Increased Expression of Gp96 by HBx-Induced NF-kB Activation Feedback Enhances Hepatitis B Virus Production
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Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer
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Blockage of Conformational Changes of Heat Shock Protein gp96 on Cell Membrane by a α-Helix Peptide Inhibits HER2 Dimerization and Signaling in Breast Cancer
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Comparative proteomic analysis of longissimus dorsi muscle in immuno-and surgically castrated male pigs
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Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer
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Comparative proteomic analysis of longissimus dorsi muscle in immuno-and surgically castrated male pigs
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Identification of potential pathways involved in the induction of cell cycle arrest and apoptosis by a new 4-arylidene curcumin analogue T63 in lung cancer cells: a comparative proteomic analysis.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.