Cytokeratin 5 (S573) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cytokeratin 5 (S573) polyclonal antibody Background :
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cytokeratin 5 (S573) polyclonal antibody detects endogenous levels of Cytokeratin 5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 550-600 of Human Cytokeratin 5. Conjugate :
Unconjugated Modification :
Unmodification
Cytokeratin 5 (S573) polyclonal antibody Background :
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratin 5 is expressed in normal basal cells. Mutations of the Cytokeratin 5 gene (KRT5) have been shown to result in the autosomal dominant disorder epidermolysis bullosa (EB). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cytokeratin 5 (S573) polyclonal antibody detects endogenous levels of Cytokeratin 5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 550-600 of Human Cytokeratin 5. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Cytokeratin 5 (S573) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Cytokeratin 5 (S573) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla
PMCID: Pubmed No.:24864235
Vitamin C Stimulates Epidermal Ceramide Production by Regulating Its Metabolic Enzymes
PMCID: Pubmed No.:26535077
LKB1 Inactivation Elicits a Redox Imbalance to Modulate Non-small Cell Lung Cancer Plasticity and Therapeutic Response.
PMCID: Pubmed No.:25936644
Vitamin C Stimulates Epidermal Ceramide Production by Regulating Its Metabolic Enzymes
PMCID: Pubmed No.:26535077
LKB1 Inactivation Elicits a Redox Imbalance to Modulate Non-small Cell Lung Cancer Plasticity and Therapeutic Response.
PMCID: Pubmed No.:25936644
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.