Product Name :
Mcl-1 (S121) polyclonal antibody Background :
B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MCL1 (S121) polyclonal antibody detects endogenous levels of MCL1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Mcl-1. Conjugate :
Unconjugated Modification :
Unmodification
Mcl-1 (S121) polyclonal antibody Background :
B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MCL1 (S121) polyclonal antibody detects endogenous levels of MCL1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human Mcl-1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Mcl-1 (S121) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of Mcl-1 (S121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Immunohistochemistry (IHC) analyzes of Mcl-1 (S121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
A novel BH3 mimetic S1 potently induces Bax/Bak-dependent apoptosis by targeting both Bcl-2 and Mcl-1
PMCID: Pubmed No.:20503275
Sorafenib Induces Growth Inhibition and Apoptosis of Human Chondrosarcoma Cells by Blocking the RAF/ERK/MEK Pathway
PMCID: Pubmed No.:20812347
Probing the difference between BH3 groove of Mcl-1 and Bcl-2 protein: Implications for dual inhibitors design
PMCID: Pubmed No.:21684048
Pan-BH3 Mimetic S1 Exhibits Broad-Spectrum Antitumor Effects by Cooperation between Bax and Bak
PMCID: Pubmed No.:23557083
iTRAQ-based quantitative proteomic analysis of the anti-apoptotic effect of hyperin, which is mediated by Mcl-1 and Bid, in H2O2-injured EA. hy926 cells
PMCID: Pubmed No.:26935776
iTRAQ-based quantitative proteomic analysis of the anti-apoptotic effect of hyperin, which is mediated by Mcl-1 and Bid, in H2O2-injured EA. hy926 cells
PMCID: Pubmed No.:26935776
iTRAQ-based quantitative proteomic analysis of the anti-apoptotic effect of hyperin, which is mediated by Mcl-1 and Bid, in H2O2-injured EA.hy926 cells
PMCID: Pubmed No.:26935776
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.