Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
MMP9 (W680) polyclonal antibody BS1241
  • Western blot (WB) analysis of MMP9 (W680) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of MMP9 (W680) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameMMP9 (W680) polyclonal antibody
Catalog No.BS1241
Swiss-ProtP14780
Host Rabbit
ReactivityHuman
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 92 kDa
Alternative NameMatrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; MMP9; CLG4B
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
Browse similar products>>
Size Price
50ul $158
100ul $275
Add to cart My orders
Product Name :
MMP9 (W680) polyclonal antibody
Background :
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or taphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is down-regulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
MMP9 (W680) polyclonal antibody detects endogenous levels of MMP9 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 650-700 of Human MMP9.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of MMP9 (W680) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate
  • Immunohistochemistry (IHC) analyzes of MMP9 (W680) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells PMCID:    Pubmed No.:22465710 Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells PMCID:    Pubmed No.:22570869 Natural Products as a Gold Mine for Selective Matrix Metalloproteinases Inhibitors PMCID:    Pubmed No.:22658537 Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways PMCID:    Pubmed No.:23026290 Protective role of metalloproteinase inhibitor (AE-941) on ulcerative colitis in rats PMCID:    Pubmed No.:23323009 The Antioxidant Property of Pachymic Acid Improves Bone Disturbance against AH Plus–induced Inflammation in MC-3T3 E1 Cells PMCID:    Pubmed No.:23522537 A novel role of ribonuclease inhibitor in regulation of epithelial-to-mesenchymal transition and ILK signaling pathway in bladder cancer cells PMCID:    Pubmed No.:23703635 Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition PMCID:    Pubmed No.:23707970 Ulinastatin Reduces Cancer Recurrence after Resection of Hepatic Metastases from Colon Cancer by Inhibiting MMP-9 Activation via the Antifibrinolytic Pathway PMCID:    Pubmed No.:23710449 Combined Inhibition of Epidermal Growth Factor Receptor and Cyclooxygenase-2 Leads to Greater Anti-tumor Activity of Docetaxel in Advanced Prostate Cancer PMCID:    Pubmed No.:24155892 Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway PMCID:    Pubmed No.:24374650 IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo PMCID:    Pubmed No.:24528069 Synergism from the combination of ulinastatin and curcumin offers greater inhibition against colorectal cancer liver metastases via modulating matrix metalloproteinase-9 and E-cadherin expression. PMCID:    Pubmed No.:24570592 Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression PMCID:    Pubmed No.:24608674 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Granulocyte/Macrophage Colony-Stimulating Factor Influences Angiogenesis by Regulating the Coordinated Expression of VEGF and the Ang/Tie System PMCID:    Pubmed No.:24658178 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Ubiquitin-Specific Peptidase USP22 Negatively Regulates the STAT Signaling Pathway by Deubiquitinating SIRT1 PMCID:    Pubmed No.:24969755 Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling. PMCID:    Pubmed No.:24912879 Effect of the PTEN gene on adhesion, invasion and metastasis of osteosarcoma cells PMCID:    Pubmed No.:25069680 Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549 PMCID:    Pubmed No.:25539072 DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling PMCID:    Pubmed No.:25520862 Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo PMCID:    Pubmed No.:25201015 Methylation of DACT2 Promotes Papillary Thyroid Cancer Metastasis by Activating Wnt Signaling PMCID:    Pubmed No.:25375359 Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. PMCID:    Pubmed No.:25377946 Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma PMCID:    Pubmed No.:25511737 Fangchinoline targets PI3K and suppresses PI3K/AKT signaling pathway in SGC7901 cells PMCID:    Pubmed No.:25872479 MicroRNA-133a inhibits cell proliferation, colony formation ability, migration and invasion by targeting matrix metallopeptidase 9 in hepatocellular carcinoma PMCID:    Pubmed No.:25607810 Ubiquitin-specific peptidase 22 inhibits colon cancer cell invasion by suppressing the signal transducer and activator of transcription 3/matrix metalloproteinase 9 pathway. PMCID:    Pubmed No.:25902005 Reno-protection of G004, a novel anti-diabetic sulfonylurea in db/db mice PMCID:    Pubmed No.:25943026 Suppression of motor protein KIF3C expression inhibits tumor growth and metastasis in breast cancer by inhibiting TGF-β signaling PMCID:    Pubmed No.:26272184 Silencing of plasminogen activator inhibitor-1 suppresses colorectal cancer progression and liver metastasis PMCID:    Pubmed No.:26275833 Holothurian glycosaminoglycan inhibits metastasis via inhibition of P-selectin in B16F10 melanoma cells PMCID:    Pubmed No.:26318439 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Effects of Methylglyoxal and Glyoxalase I Inhibition on Breast Cancer Cells Proliferation, Invasion, and Apoptosis through Modulation of MAPKs, MMP9, and Bcl-2. PMCID:    Pubmed No.:26618552 Epb41l3 suppresses esophageal squamous cell carcinoma invasion and inhibits MMP2 and MMP9 expression PMCID:    Pubmed No.:26916087 Neotuberostemonine attenuates bleomycin-induced pulmonary fibrosis by suppressing the recruitment and activation of macrophages PMCID:    Pubmed No.:27144994 Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549 PMCID:    Pubmed No.:25539072 DACT2 is frequently methylated in human gastric cancer and methylation of DACT2 activated Wnt signaling PMCID:    Pubmed No.:25520862 Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo PMCID:    Pubmed No.:25201015 Methylation of DACT2 Promotes Papillary Thyroid Cancer Metastasis by Activating Wnt Signaling PMCID:    Pubmed No.:25375359 Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits. PMCID:    Pubmed No.:25377946 Toll like receptor 4 facilitates invasion and migration as a cancer stem cell marker in hepatocellular carcinoma PMCID:    Pubmed No.:25511737 Combined Inhibition of Epidermal Growth Factor Receptor and Cyclooxygenase-2 Leads to Greater Anti-tumor Activity of Docetaxel in Advanced Prostate Cancer PMCID:    Pubmed No.:24155892 Lipoxin A4 Attenuates Cell Invasion by Inhibiting ROS/ERK/MMP Pathway in Pancreatic Cancer PMCID:    Pubmed No.:26649143 Effects of Methylglyoxal and Glyoxalase I Inhibition on Breast Cancer Cells Proliferation, Invasion, and Apoptosis through Modulation of MAPKs, MMP9, and Bcl-2. PMCID:    Pubmed No.:26618552 Epb41l3 suppresses esophageal squamous cell carcinoma invasion and inhibits MMP2 and MMP9 expression PMCID:    Pubmed No.:26916087 Role of cathepsin B in regulating migration and invasion of fibroblast‐like synoviocytes into inflamed tissue from patients with rheumatoid arthritis PMCID:    Pubmed No.:24749816 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 Poloxamer-188 Can Attenuate Blood–Brain Barrier Damage to Exert Neuroprotective Effect in Mice Intracerebral Hemorrhage Model PMCID:    Pubmed No.:24770901 Epigenetic silencing of SFRP1 and SFRP5 by hepatitis B virus X protein enhances hepatoma cell tumorigenicity through Wnt signaling pathway PMCID:    Pubmed No.:24374650 IDH1 Overexpression Induced Chemotherapy Resistance and IDH1 Mutation Enhanced Chemotherapy Sensitivity in Glioma Cells in Vitro and in Vivo PMCID:    Pubmed No.:24528069 Synergism from the combination of ulinastatin and curcumin offers greater inhibition against colorectal cancer liver metastases via modulating matrix metalloproteinase-9 and E-cadherin expression. PMCID:    Pubmed No.:24570592 Icaritin suppresses the proliferation of human osteosarcoma cells in vitro by increasing apoptosis and decreasing MMP expression PMCID:    Pubmed No.:24608674 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Granulocyte/Macrophage Colony-Stimulating Factor Influences Angiogenesis by Regulating the Coordinated Expression of VEGF and the Ang/Tie System PMCID:    Pubmed No.:24658178 AEG‐1 induces gastric cancer metastasis by upregulation of eIF4E expression PMCID:    Pubmed No.:28661037 lncRNA H19 regulates epithelial–mesenchymal transition and metastasis of bladder cancer by miR-29b-3p as competing endogenous RNA PMCID:    Pubmed No.:28779971 PT93, a novel caffeic acid amide derivative, suppresses glioblastoma cells migration, proliferation and MMP-2/-9 expression PMCID:    Pubmed No.:28454354 Baicalein, unlike 4-hydroxytamoxifen but similar to G15, suppresses 17β-estradiol-induced cell invasion, and matrix metalloproteinase-9 expression and activation in MCF-7 human breast cancer cells PMCID:    Pubmed No.:28789417 All-trans retinoic acid inhibits migration, invasion and proliferation, and promotes apoptosis in glioma cells in vitro PMCID:    Pubmed No.:26137156 miR-600 inhibits cell proliferation, migration and invasion by targeting p53 in mutant p53-expressing human colorectal cancer cell lines PMCID:    Pubmed No.:28454325 Effects of pamidronate disodium on the loss of osteoarthritic subchondral bone and the expression of cartilaginous and subchondral osteoprotegerin and RANKL in rabbits PMCID:    Pubmed No.:25377946 Ubiquitin-specific peptidase 22 inhibits colon cancer cell invasion by suppressing the signal transducer and activator of transcription 3/matrix metalloproteinase 9 pathway PMCID:    Pubmed No.:25902005 Targeted knockout of TNF‑α by injection of lentivirus‑mediated siRNA into the subacromial bursa for the treatment of subacromial bursitis in rats PMCID:    Pubmed No.:26130073 Emodin inhibits migration and invasion of MHCC‑97H human hepatocellular carcinoma cells PMCID:    Pubmed No.:27882165 Methylation of DACT2 accelerates esophageal cancer development by activating Wnt signaling PMCID:    Pubmed No.:26919254 Methylation of NRN1 is a novel synthetic lethal marker of PI3K‐Akt‐mTOR and ATR inhibitors in esophageal cancer PMCID:    Pubmed No.:33931924 Long non-coding RNA MCM3AP-AS1 promotes growth and migration through modulating FOXK1 by sponging miR-138-5p in pancreatic cancer PMCID:    Pubmed No.:31830901 Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma PMCID:    Pubmed No.:30400968 Silencing of KIF3B Suppresses Breast Cancer Progression by Regulating EMT and Wnt/β-Catenin Signaling PMCID:    Pubmed No.:33542902 Depletion of VPS35 attenuates metastasis of hepatocellular carcinoma by restraining the Wnt/PCP signaling pathway PMCID:    Pubmed No.:33997170 Chinese herbal formula QHF inhibits hepatocellular carcinoma metastasis via HGF/c-Met signaling pathway PMCID:    Pubmed No.:33075668 Chinese herbal formula QHF inhibits hepatocellular carcinoma metastasis via HGF/c-Met signaling pathway PMCID:    Pubmed No.:33075668 c‐Fos separation from Lamin A/C by GDF15 promotes colon cancer invasion and metastasis in inflammatory microenvironment PMCID:    Pubmed No.:31613004 YiQiFuMai Lyophilized Injection ameliorates tPA-induced hemorrhagic transformation by inhibiting cytoskeletal rearrangement associated with ROCK1 and NF-κB signaling pathways PMCID:    Pubmed No.:32730882 Discovery of a novel rhein-SAHA hybrid as a multi-targeted anti-glioblastoma drug PMCID:    Pubmed No.:31414267 Long noncoding RNA NEAT1 promotes cell proliferation, migration, and invasion in hepatocellular carcinoma through interacting with miR‐384 PMCID:    Pubmed No.:30346062 Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation PMCID:    Pubmed No.:31572058 Tumor-secreted GRP78 facilitates the migration of macrophages into tumors by promoting cytoskeleton remodeling PMCID:    Pubmed No.:30959099 Neurovascular integrative effects of long-term environmental enrichment on chronic cerebral hypoperfusion rat model PMCID:    Pubmed No.:32711044 TMEFF2 inhibits pancreatic cancer cells proliferation, migration, and invasion by suppressing phosphorylation of the MAPK signaling pathway PMCID:    Pubmed No.:31920328 The Long Noncoding RNA LOXL1-AS1 Promotes the Proliferation, Migration, and Invasion in Hepatocellular Carcinoma PMCID:    Pubmed No.:33381389 Pectin Oligosaccharides Enhance α2,6-Sialylation Modification that Promotes Apoptosis of Bladder Cancer Cells by Targeting the Hedgehog Pathway PMCID:    Pubmed No.:34041669 UBE2W down‐regulation promotes cell apoptosis and correlates with hypospermatogenesis PMCID:    Pubmed No.:31710394 Downregulation of Astrocyte Elevated Gene-1 Expression Combined with All-Trans Retinoic Acid Inhibits Development of Vasculogenic Mimicry and Angiogenesis in Glioma PMCID:    Pubmed No.:35201552
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER