NSE/Enolase (R400) polyclonal antibody 
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Product Name :
NSE/Enolase (R400) polyclonal antibody Background :
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric euroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NSE/ENO2 (R400) polyclonal antibody detects endogenous levels of NSE/ENO2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 361-410 of Human NSE/Enolase. Conjugate :
Unconjugated Modification :
Unmodification
NSE/Enolase (R400) polyclonal antibody Background :
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric euroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NSE/ENO2 (R400) polyclonal antibody detects endogenous levels of NSE/ENO2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 361-410 of Human NSE/Enolase. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NSE/Enolase (R400) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:The brain tissue lysate of Mouse(40ug) Lane3:The brain tissue lysate of Rat(40ug) Lane4:The lung tissue lysate of Mouse(40ug) -
Immunohistochemistry (IHC) analyzes of NSE/Enolase (R400) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.