p15 INK4b (G113) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
p15 INK4b (G113) polyclonal antibody Background :
p15 (PAF), also known as PAF, L5, OEATC1 (overexpressed in anaplastic thyroid carcinoma 1) or NS5ATP9, is a 111 amino acid protein that localizes to both the nucleus and the mitochondria. Highly expressed in colon and thymus with lower expression in liver, ovary, kidney, spleen, placenta and small intestine, p15 (PAF) interacts with the nuclear antigen PCNA and, through this interaction, is thought to protect cells from UV-induced cell death. The association of p15 (PAF) and PCNA is enhanced by UV treatment and is facilitated by the binding of ING1, a tumor suppressor that can induce apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p15 INK4b (G113) polyclonal antibody detects endogenous levels of p15 INK4b protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human p15 INK4b. Conjugate :
Unconjugated Modification :
Unmodification
p15 INK4b (G113) polyclonal antibody Background :
p15 (PAF), also known as PAF, L5, OEATC1 (overexpressed in anaplastic thyroid carcinoma 1) or NS5ATP9, is a 111 amino acid protein that localizes to both the nucleus and the mitochondria. Highly expressed in colon and thymus with lower expression in liver, ovary, kidney, spleen, placenta and small intestine, p15 (PAF) interacts with the nuclear antigen PCNA and, through this interaction, is thought to protect cells from UV-induced cell death. The association of p15 (PAF) and PCNA is enhanced by UV treatment and is facilitated by the binding of ING1, a tumor suppressor that can induce apoptosis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p15 INK4b (G113) polyclonal antibody detects endogenous levels of p15 INK4b protein. Immunogen :
Synthetic peptide, corresponding to amino acids 100-150 of Human p15 INK4b. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of p15 INK4b (G113) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate -
Immunohistochemistry (IHC) analyzes of p15 INK4b (G113) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.