Product Name :
p53 (S9) polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 (S9) polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human p53. Conjugate :
Unconjugated Modification :
Unmodification
p53 (S9) polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 (S9) polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Synthetic peptide, corresponding to the N-terminus of Human p53. Conjugate :
Unconjugated Modification :
Unmodification
-
-
Immunohistochemistry (IHC) analyzes of p53 (S9) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Targeted delivery of a novel palmitylated D-peptide for antiglioblastoma molecular therapy
PMCID: Pubmed No.:22233211
Down regulation of differentiated embryonic chondrocytes 1 (DEC1) is involved in 8-methoxypsoralen-induced apoptosis in HepG2 cells
PMCID: Pubmed No.:22796345
The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines
PMCID: Pubmed No.:23200181
Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway
PMCID: Pubmed No.:24213358
Integration of microarray profiles associated with cardiomyopathy and the potential role of Ube3a in apoptosis
PMCID: Pubmed No.:24337433
Qi-Dan Fang ameliorates adriamycin-induced nephrotic syndrome rat model by enhancing renal function and inhibiting podocyte injury
PMCID: Pubmed No.:24389029
TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway
PMCID: Pubmed No.:24469053
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
Radiosensitization to X-ray radiation by telomerase inhibitor MST-312 in human hepatoma HepG2 cells
PMCID: Pubmed No.:25596016
Radiosensitization to X-ray radiation by telomerase inhibitor MST-312 in human hepatoma HepG2 cells
PMCID: Pubmed No.:25596016
Triptolide induces apoptosis in endometrial cancer via a p53‑independent mitochondrial pathway
PMCID: Pubmed No.:24213358
Integration of microarray profiles associated with cardiomyopathy and the potential role of Ube3a in apoptosis
PMCID: Pubmed No.:24337433
Qi-Dan Fang ameliorates adriamycin-induced nephrotic syndrome rat model by enhancing renal function and inhibiting podocyte injury
PMCID: Pubmed No.:24389029
TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway
PMCID: Pubmed No.:24469053
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
The co‑treatment of metformin with flavone synergistically induces apoptosis through inhibition of PI3K/AKT pathway in breast cancer cells
PMCID: Pubmed No.:29552226
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.