Product Name :
p63 (N662) polyclonal antibody Background :
The p53 gene is a widely studied anti-oncogene, or tumor suppressor gene. The p53 gene product can act as a negative regulator of cell growth in response to DNA damage. p73 shares a high degree of homology with p53, and appears to have similar growth-inhibiting and apoptosis-promoting functions. However, unlike p53, the expression of p73 is not upregulated in response to DNA damage. p73 can, when overproduced, activate the p53- responsive gene p21. p63 has also been identified based on its similarities with p53. The p63 gene encodes multiple isotypes with variable functions. p63α (also designated p51B or KET), p63β and p63γ (also designated p51A), as well as corresponding TA* p63 isoforms, contain transactivation domains which have been shown to transactivate p53 reporter genes and induce apoptosis. p63 isoforms lack the transactivation domain and can act as dominant- negative reagents to inhibit transactivation by p53 and p63. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p63 (N662) polyclonal antibody detects endogenous levels of p63 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 634-680 of Human p63. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of p63 (N662) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
• 
  Immunohistochemistry (IHC) analyzes of p63 (N662) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.showing nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---