Product Name :
RARβ (L359) polyclonal antibody Background :
Retinoids are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. Retinoic acid receptors (RARs) and retinoid X receptors (RXRs) are nuclear transcription factors that modulate the effects of retinoids (RA) on gene expression. Most retinoid forms (including all trans RA, 9-cis RA, 4oxo RA and 3,4 dihydro RA) activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RA binds RARs, inducing a change in receptor configuration that allows DNA binding and increased gene transcription from specific genes to occur. RAR family members, which include RARα, RARβ and RARγ, belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. Retinoid receptor expression is tissue specific; the skin expresses RARγ and RXRα. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Retinoic Acid Receptor β (L359) polyclonal antibody detects endogenous levels of Retinoic Acid Receptor β protein. Immunogen :
Synthetic peptide, corresponding to amino acids 321-370 of Human RARβ. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RARβ (L359) pAb at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of RARβ (L359) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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