Product Name :
Tau (V256) polyclonal antibody Background :
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tau (V256) polyclonal antibody detects endogenous levels of Tau protein. Immunogen :
Synthetic peptide, corresponding to amino acids 221-270 of Human Tau. Conjugate :
Unconjugated Modification :
Unmodification
Tau (V256) polyclonal antibody Background :
Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Tau (V256) polyclonal antibody detects endogenous levels of Tau protein. Immunogen :
Synthetic peptide, corresponding to amino acids 221-270 of Human Tau. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Tau (V256) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:U-251 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of Tau (V256) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Trio Is a Key Guanine Nucleotide Exchange Factor Coordinating Regulation of theMigration andMorphogenesis of Granule Cells in the Developing Cerebellum
PMCID: Pubmed No.:20516067
Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats
PMCID: Pubmed No.:24337945
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
The relationships between the chemosensitivity of human gastric cancer to paclitaxel and the expressions of class III β-tubulin, MAPT, and survivin
PMCID: Pubmed No.:24722794
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
Proteasome Inhibition-Induced Downregulation of Akt/GSK-3β Pathway Contributes to Abnormality of Tau in Hippocampal Slice
PMCID: Pubmed No.:24752588
Effect of naringenin on brain insulin signaling and cognitive functions in ICV-STZ induced dementia model of rats
PMCID: Pubmed No.:24337945
UCH-L1 Inhibition Involved in CREB Dephosphorylation in Hippocampal Slices
PMCID: Pubmed No.:24323362
The relationships between the chemosensitivity of human gastric cancer to paclitaxel and the expressions of class III β-tubulin, MAPT, and survivin
PMCID: Pubmed No.:24722794
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.