Product Name :
TGFβ2 (L388) polyclonal antibody Background :
Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGFβ requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGFβ3 protein has approximately 80% identity to the mature region of both TGFβ1 and TGFβ2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TGF β2 (L388) polyclonal antibody detects endogenous levels of TGF β2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 360-400 of Human TGFβ2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TGFβ2 (L388) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
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  Immunohistochemistry (IHC) analyzes of TGFβ2 (L388) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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