PYK2 (I574) polyclonal antibody 
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Product Name :
PYK2 (I574) polyclonal antibody Background :
Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PYK2 (I574) polyclonal antibody detects endogenous levels of PYK2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1. Conjugate :
Unconjugated Modification :
Unmodification
PYK2 (I574) polyclonal antibody Background :
Pyk2 is a protein tyrosine kinase related to Focal Adhesion Kinase (p125 FAK). Pyk2 is highly expressed in the central nervous system. It is phosphorylated on tyrosine residues in response to various stimuli that elevate intracellular calcium concentration, as well as by protein kinase C activation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PYK2 (I574) polyclonal antibody detects endogenous levels of PYK2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PYK2 (I574) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:The Brain tissue lysate of Mouse (40ug) Lane5:The Brain tissue lysate of Rat(40ug) -
Immunohistochemistry (IHC) analyzes of PYK2 (I574) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.