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Vimentin (I444) polyclonal antibody BS1491
  • Western blot (WB) analysis of Vimentin (I444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2
  • Immunohistochemistry (IHC) analyzes of Vimentin (I444) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameVimentin (I444) polyclonal antibody
Catalog No.BS1491
Swiss-ProtP08670
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 40, 57 kDa
Alternative NameVimentin; VIM
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $158
100ul $275
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Product Name :
Vimentin (I444) polyclonal antibody
Background :
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. Intermediate filaments are constructed from two-chain, α-helical, coiled-coil molecules arranged on an imperfect helical lattice and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. One such intermediate filament protein, Vimentin, is a general marker of cells originating in the mesenchyme. Vimentin is frequently coexpressed with other members of the intermediate filament family, such as the cytokeratins, in neoplasms including melanoma and breast carcinoma.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Vimentin (I444) polyclonal antibody detects endogenous levels of Vimentin protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 411-460 of Human Vimentin.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Vimentin (I444) polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2
  • Immunohistochemistry (IHC) analyzes of Vimentin (I444) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Up-regulating ribonuclease inhibitor inhibited epithelial-to-mesenchymal transition and metastasis in murine melanoma cells PMCID:    Pubmed No.:22465710 Inhibition of hypoxia-induced epithelial mesenchymal transition by luteolin in non-small cell lung cancer cells. PMCID:    Pubmed No.:22552526 Downregulation of integrin-linked kinase inhibits epithelial-to-mesenchymal transition and metastasis in bladder cancer cells PMCID:    Pubmed No.:22570869 Neuropilin-1 down-regulation impairs cell migration and induces the differentiation of human tongue squamous cell carcinoma cells. PMCID:    Pubmed No.:23104536 miR-612 suppresses the invasive-metastatic cascade in hepatocellular carcinoma. PMCID:    Pubmed No.:23478189 MicroRNA-21 suppresses PTEN and hSulf-1 expression and promotes hepatocellular carcinoma progression through AKT/ERK pathways PMCID:    Pubmed No.:23684551 Critical Roles of p53 in Epithelial-Mesenchymal Transition and Metastasis of Hepatocellular Carcinoma Cells PMCID:    Pubmed No.:24023784 LncRNA expression signatures of twist-induced epithelial-to-mesenchymal transition in MCF10A cells PMCID:    Pubmed No.:24113349 Epigenetic Silencing of CXCL14 Induced Colorectal Cancer Migration and Invasion PMCID:    Pubmed No.:24099668 Transcatheter arterial embolization promotes liver tumor metastasis by increasing the population of circulating tumor cells PMCID:    Pubmed No.:24235842 Effect of downregulation of ZEB1 on vimentin expression, tumor migration and tumorigenicity of melanoma B16F10 cells and CSCs PMCID:    Pubmed No.:24339410 TGFbetaR2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness PMCID:    Pubmed No.:24606633 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Evidence of TGF-β 1 mediated epithelial-mesenchymal transition in immortalized benign prostatic hyperplasia cells PMCID:    Pubmed No.:24650126 Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling PMCID:    Pubmed No.:24686087 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 PMCID:    Pubmed No.:24812621 Protein Tyrosine Phosphatase U (PTPRU) is Required for Glioma Growth and Motility PMCID:    Pubmed No.:24876153 Morphology and functions of astrocytes cultured on water-repellent fractal tripalmitin surfaces PMCID:    Pubmed No.:24894646 Epigenetic silencing of DACH1 induces the invasion and metastasis of gastric cancer by activating TGF-β signalling. PMCID:    Pubmed No.:24912879 Gastric cancer-derived MSC-secreted PDGF-DD promotes gastric cancer progression. PMCID:    Pubmed No.:24938433 Neuropilin-1 Promotes Epithelial-to-Mesenchymal Transition by Stimulating Nuclear Factor-Kappa B and Is Associated with Poor Prognosis in Human Oral Squamous Cell Carcinoma. PMCID:    Pubmed No.:24999732 FOXC2 promotes colorectal cancer metastasis by directly targeting MET PMCID:    Pubmed No.:25381815 Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer PMCID:    Pubmed No.:25546612 HES1 promotes metastasis and predicts poor survival in patients with colorectal cancer PMCID:    Pubmed No.:25636905 Malic enzyme 1 induces epithelial-mesenchymal transition and indicates poor prognosis in hepatocellular carcinoma. PMCID:    Pubmed No.:25753478 Overexpressed GRP78 affects EMT and cell-matrix adhesion via autocrine TGF-β/Smad2/3 signaling PMCID:    Pubmed No.:25934251 Specific chemotherapeutic agents induce metastatic behaviour through stromal‐and tumour‐derived cytokine and angiogenic factor signalling PMCID:    Pubmed No.:25988668 The effect of TGF-beta-induced epithelial-mesenchymal transition on the expression of intracellular calcium-handling proteins in T47D and MCF-7 human breast cancer cells. PMCID:    Pubmed No.:26247838 Suppression of motor protein KIF3C expression inhibits tumor growth and metastasis in breast cancer by inhibiting TGF-β signaling PMCID:    Pubmed No.:26272184 Curcumin inhibits cobalt chloride-induced epithelial-to-mesenchymal transition associated with interference with TGF-β/Smad signaling in hepatocytes PMCID:    Pubmed No.:26302188 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway PMCID:    Pubmed No.:26418365 Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma PMCID:    Pubmed No.:26549030 Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells PMCID:    Pubmed No.:27069543 Cancer stem cell vaccine expressing ESAT-6-gpi and IL-21 inhibits melanoma growth and metastases PMCID:    Pubmed No.:26692931 Silencing of HMGA2 suppresses cellular proliferation, migration, invasion, and epithelial–mesenchymal transition in bladder cancer PMCID:    Pubmed No.:26684800 Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells PMCID:    Pubmed No.:26741501 Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal PMCID:    Pubmed No.:27017226 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 FOXC2 promotes colorectal cancer metastasis by directly targeting MET PMCID:    Pubmed No.:25381815 Cell membrane gp96 facilitates HER2 dimerization and serves as a novel target in breast cancer PMCID:    Pubmed No.:25546612 LncRNA expression signatures of twist-induced epithelial-to-mesenchymal transition in MCF10A cells PMCID:    Pubmed No.:24113349 Epigenetic Silencing of CXCL14 Induced Colorectal Cancer Migration and Invasion PMCID:    Pubmed No.:24099668 Transcatheter arterial embolization promotes liver tumor metastasis by increasing the population of circulating tumor cells PMCID:    Pubmed No.:24235842 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 LncRNA‐Hh Strengthen Cancer Stem Cells Generation in Twist‐Positive Breast Cancer via Activation of Hedgehog Signaling Pathway PMCID:    Pubmed No.:26418365 Hepatitis C virus core protein interacts with Snail and histone deacetylases to promote the metastasis of hepatocellular carcinoma PMCID:    Pubmed No.:26549030 Decreasing lncRNA HOTAIR expression inhibits human colorectal cancer stem cells PMCID:    Pubmed No.:27069543 Cancer stem cell vaccine expressing ESAT-6-gpi and IL-21 inhibits melanoma growth and metastases PMCID:    Pubmed No.:26692931 Silencing of HMGA2 suppresses cellular proliferation, migration, invasion, and epithelial–mesenchymal transition in bladder cancer PMCID:    Pubmed No.:26684800 Oroxylin A inhibits invasion and migration through suppressing ERK/GSK‐3β signaling in snail‐expressing non‐small‐cell lung cancer cells PMCID:    Pubmed No.:26741501 Gastrointestinal Factor GDDR Attenuates Epithelial–Mesenchymal Transition in Gastric Cancer via Inhibiting AKT Signal PMCID:    Pubmed No.:27017226 Reinforcing B16F10/GPI-IL-21 vaccine efficacy against melanoma by injecting mice with shZEB1 plasmid or miR200c agomir PMCID:    Pubmed No.:25895132 Down-regulating ribonuclease inhibitor enhances metastasis of bladder cancer cells through regulating epithelial-mesenchymal transition and ILK signaling pathway PMCID:    Pubmed No.:24768914 PMCID:    Pubmed No.:24812621 Effect of downregulation of ZEB1 on vimentin expression, tumor migration and tumorigenicity of melanoma B16F10 cells and CSCs PMCID:    Pubmed No.:24339410 TGFbetaR2 is a major target of miR-93 in nasopharyngeal carcinoma aggressiveness PMCID:    Pubmed No.:24606633 Regulation gene expression of miR200c and ZEB1 positively enhances effect of tumor vaccine B16F10/GPI-IL-21 on inhibition of melanoma growth and metastasis PMCID:    Pubmed No.:24625224 Oroxylin A inhibits hypoxia-induced invasion and migration of MCF-7 cells by suppressing the Notch pathway PMCID:    Pubmed No.:24637576 Evidence of TGF-β 1 mediated epithelial-mesenchymal transition in immortalized benign prostatic hyperplasia cells PMCID:    Pubmed No.:24650126 Pyruvate kinase M2 facilitates colon cancer cell migration via the modulation of STAT3 signalling PMCID:    Pubmed No.:24686087 Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1- PMCID:    Pubmed No.:25990368 Neuronal mTORC1 Is Required for Maintaining the Nonreactive State of Astrocytes PMCID:    Pubmed No.:27895121 Biological roles of human bone morphogenetic protein 9 in the bone microenvironment of human breast cancer MDA-MB-231 cells PMCID:    Pubmed No.:26550465 CUL4A facilitates hepatocarcinogenesis by promoting cell cycle progression and epithelial-mesenchymal transition PMCID:    Pubmed No.:26593394 miR520c blocks EMT progression of human breast cancer cells by repressing STAT3 PMCID:    Pubmed No.:28112380 Exosomes derived from human mesenchymal stem cells promote gastric cancer cell growth and migration via the activation of the Akt pathway PMCID:    Pubmed No.:27513187 Endogenous Nampt upregulation is associated with diabetic nephropathy inflammatory‑fibrosis through the NF‑κB p65 and Sirt1 pathway; NMN alleviates diabetic nephropathy inflammatory‑fibrosis by inhibiting endogenous Nampt PMCID:    Pubmed No.:29104634 Silencing of KIF3B Suppresses Breast Cancer Progression by Regulating EMT and Wnt/β-Catenin Signaling PMCID:    Pubmed No.:33542902 Although c‑MYC contributes to tamoxifen resistance, it improves cisplatin sensitivity in ER‑positive breast cancer PMCID:    Pubmed No.:32319562 The anti‑migration and anti‑invasion effects of Bruceine D in human triple‑negative breast cancer MDA‑MB‑231 cells PMCID:    Pubmed No.:31853299 Protective effects of heterophyllin B against bleomycin-induced pulmonary fibrosis in mice via AMPK activation PMCID:    Pubmed No.:35283110 Simiao pill inhibits epithelial mesenchymal transition in a mouse model of chronic hyperuricemic nephropathy by inhibiting NLRP3 inflammasome activation PMCID:    Pubmed No.:36271349 MiR-138-5p suppresses the progression of lung cancer by targeting SNIP1 PMCID:    Pubmed No.:36597175 Cigarette Smoke-Induced Gastric Cancer Cell Exosomes Affected the Fate of Surrounding Normal Cells via the Circ0000670/Wnt/β-Catenin Axis PMCID:    Pubmed No.:37235279 Exosomal hsa_circ_000200 as a potential biomarker and metastasis enhancer of gastric cancer via miR-4659a/b-3p/HBEGF axis PMCID:    Pubmed No.:37525152
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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