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Bcl-2 (P65) polyclonal antibody BS1511
  • Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549
  • Immunohistochemistry (IHC) analyzes of Bcl-2 (P65) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Product NameBcl-2 (P65) polyclonal antibody
Catalog No.BS1511
Swiss-ProtP10415
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 26 kDa
Alternative NameApoptosis regulator Bcl-2; Bcl 2; BCL2
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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50ul $158
100ul $275
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Product Name :
Bcl-2 (P65) polyclonal antibody
Background :
Bcl-2 is one among many key regulators of apoptosis which are essential for proper development, tissue homeostasis and protection against foreign pathogens. Human Bcl-2 is a 26 kDa, membraneassociated, anti-apoptotic oncoprotein that can promote cell survival through protein-protein interactions with other Bcl-2 related family members, such as the death suppressors Bcl-xL, Mcl-1, Bcl-w and A1, or the death agonists Bax, Bak, Bik, Bad and BID. The anti-apoptotic function of Bcl-2 can also be regulated through proteolytic processing and phosphorylation. Bcl-2 may promote cell survival by interfering with the activation of the cytochrome c/Apaf-1 pathway through stabilization of the mitochondrial membrane.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
Bcl-2 (P65) polyclonal antibody detects endogenous levels of Bcl-2 protein.
Immunogen :
Synthetic peptide, corresponding to amino acids 31-80 of Human Bcl-2.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot (WB) analysis of Bcl-2 (P65) polyclonal antibody at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:A549
  • Immunohistochemistry (IHC) analyzes of Bcl-2 (P65) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Adiponectin reduces ER stress-induced apoptosis through PPAR α transcriptional regulation of ATF2 in mouse adipose PMCID:    Pubmed No.:27882945 miR-181b modulates multidrug resistance by targeting BCL2 in human cancer cell lines PMCID:    Pubmed No.:20162574 Involvement of JNK and P53 Activation in G2/M Cell Cycle Arrest and Apoptosis induced by Titanium Dioxide Nanoparticles in neuron cells PMCID:    Pubmed No.:20863874 Small interfering RNA targeting integrin-linked kinase inhibited the growth and induced apoptosis in human bladder cancer cells PMCID:    Pubmed No.:21601006 Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by α4 Protein in HEK293 Cells PMCID:    Pubmed No.:21698000 Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells PMCID:    Pubmed No.:21727907 Luteolin protects against reactive oxygen species-mediated cell death induced by zinc toxicity via the PI3K–Akt–NF-κB–ERK-dependent pathway PMCID:    Pubmed No.:21800350 2,5-Hexanedione induces human ovarian granulosa cell apoptosis through BCL-2, BAX, and CASPASE-3 signaling pathways PMCID:    Pubmed No.:21901545 Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy PMCID:    Pubmed No.:21980237 miR-200bc/429 cluster modulates multidrug resistance of human cancer cell lines by targeting BCL2 and XIAP PMCID:    Pubmed No.:21993663 Inhibition of activated Stat3 reverses drug resistance to chemotherapeutic agents in gastric cancer cells PMCID:    Pubmed No.:22104727 (S)-ZJM-289, a nitric oxide-releasing derivative of 3-n-butylphthalide, protects against ischemic neuronal injury by attenuating mitochondrial dysfunction and associated cell death PMCID:    Pubmed No.:22142531 Treatment With Ginseng Total Saponins Reduces the Secondary Brain Injury in Rat After Cortical Impact PMCID:    Pubmed No.:22434648 A natural coumarin derivative esculetin offers neuroprotection on cerebral ischemia/reperfusion injury in mice PMCID:    Pubmed No.:22458555 Apple polysaccharides induce apoptosis in colorectal cancer cells. PMCID:    Pubmed No.:22470124 Curcumin inhibits tumor proliferation induced by neutrophil elastase through the upregulation of a1-antitrypsin in lung cancer PMCID:    Pubmed No.:22507634 Microcystin-LR Induces Ceramide to Regulate PP2A and Destabilize Cytoskeleton in HEK293 Cells PMCID:    Pubmed No.:22523228 The role of the IRE1 pathway in PBDE-47-induced toxicity in human neuroblastoma SH-SY5Y cells in vitro PMCID:    Pubmed No.:22543052 miR-181a/b significantly enhances drug sensitivity in chronic lymphocytic leukemia cells via targeting multiple anti-apoptosis genes PMCID:    Pubmed No.:22610076 Reactive oxygen species-mediated mitochondrial pathway is involved in Baohuoside I-induced apoptosis in human non-small cell lung cancer PMCID:    Pubmed No.:22687635 Activation of nuclear factor erythroid 2-related factor 2 and PPARγ plays a role in the genistein-mediated attenuation of oxidative stress-induced endothelial cell injury PMCID:    Pubmed No.:22716961 Down regulation of differentiated embryonic chondrocytes 1 (DEC1) is involved in 8-methoxypsoralen-induced apoptosis in HepG2 cells PMCID:    Pubmed No.:22796345 Methylated actinomycin D, a novel actinomycin D analog induces apoptosis in HepG2 cells through fas- and mitochondria-mediated pathways† PMCID:    Pubmed No.:22821714 Impaired cardiac microvascular endothelial cells function induced by Coxsackievirus B3 infection and its potential role in cardiac fibrosis PMCID:    Pubmed No.:22867880 LYG-202 Augments Tumor Necrosis Factor-α-induced Apoptosis Via Attenuating CK2-dependent Nuclear Factor-κB Pathway in HepG2 Cells PMCID:    Pubmed No.:22909797 Akebia Saponin D attenuates amyloid β-induced cognitive deficits and inflammatory response in rats: Involvement of Akt/NF-κB pathway PMCID:    Pubmed No.:22963993 Neurotoxic potential of iron oxide nanoparticles in the rat brain striatum and hippocampus PMCID:    Pubmed No.:22995439 Phycocyanin may suppress d-galactose-induced human lens epithelial cell apoptosis through mitochondrial and unfolded protein response pathways PMCID:    Pubmed No.:23036742 ZFX regulates glioma cell proliferation and survival in vitro and in vivo PMCID:    Pubmed No.:23322077 miR-17-5p/20a are important markers for gastric cancer and murine double minute 2 participates in their functional regulation PMCID:    Pubmed No.:23333058 Phosphorylated Hsp27 activates ATM-dependent p53 signaling and mediates the resistance of MCF-7 cells to doxorubicin-induced apoptosis PMCID:    Pubmed No.:23357534 A Histone Acetyltransferase p300 Inhibitor C646 Induces Cell Cycle Arrest and Apoptosis Selectively in AML1-ETO-Positive AML Cells PMCID:    Pubmed No.:23390536 Functional graphene oxide as a plasmid-based Stat3 siRNA carrier inhibits mouse malignant melanoma growth in vivo PMCID:    Pubmed No.:23425941 S-allyl cysteine restores erectile function through inhibition of reactive oxygen species generation in diabetic rats PMCID:    Pubmed No.:23427186 [Gly14]-Humanin Offers Neuroprotection through Glycogen Synthase Kinase-3β Inhibition in a Mouse Model of Intracerebral Hemorrhage PMCID:    Pubmed No.:23538063 Paclitaxel-Fe3O4 nanoparticles inhibit growth of CD138- CD34- tumor stem-like cells in multiple myeloma-bearing mice PMCID:    Pubmed No.:23610522 Effect of bone marrow mesenchymal stem cells from blastic phase chronic myelogenous leukemia on the growth and apoptosis of leukemia cells PMCID:    Pubmed No.:23733230 Proton pump inhibitor selectively suppresses proliferation and restores the chemosensitivity of gastric cancer cells by inhibiting STAT3 signaling pathway PMCID:    Pubmed No.:23973653 ONTD induces apoptosis of human hepatoma Bel-7402 cells via a MAPK-dependent mitochondrial pathway and the depletion of intracellular glutathione PMCID:    Pubmed No.:24036456 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:None MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:24335166 Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats PMCID:    Pubmed No.:24613208 Overexpression of BAT3 Alleviates Prion Protein Fragment PrP106‐126‐Induced Neuronal Apoptosis PMCID:    Pubmed No.:24629137 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Exogenous High Mobility Group Box 1 Inhibits Apoptosis and Promotes the Proliferation of Lewis Cells via RAGE/TLR4‐Dependent Signal Pathways PMCID:    Pubmed No.:24673192 Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway PMCID:    Pubmed No.:25213258 Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways PMCID:    Pubmed No.:25270871 Involvement of miR-143 in cisplatin resistance of gastric cancer cells via targeting IGF1R and BCL2 PMCID:    Pubmed No.:25492481 MicroRNA-181 functions as a tumor suppressor in non-small cell lung cancer (NSCLC) by targeting Bcl-2 PMCID:    Pubmed No.:25524579 Huangzhi Oral Liquid Prevents Arrhythmias by Upregulating Caspase-3 and Apoptosis Network Proteins in Myocardial Ischemia-Reperfusion Injury in Rats PMCID:    Pubmed No.:26074995 Activation of cellular apoptosis in the cecal epithelium is associated with increased oxidative reactions in lactating goats following high concentrate diet feeding PMCID:    Pubmed No.:25590230 LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells PMCID:    Pubmed No.:25945460 8-Methoxypsoralen Induces Intrinsic Apoptosis in HepG2 Cells: Involvement of Reactive Oxygen Species Generation and ERK1/2 Pathway Inhibition PMCID:    Pubmed No.:26316083 Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO 2-induced senescent mice PMCID:    Pubmed No.:27064004 PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells PMCID:    Pubmed No.:26860588 Blocking B7-1/CD28 Pathway Diminished Long-Range Brain Damage by Regulating the Immune and Inflammatory Responses in a Mouse Model of Intracerebral Hemorrhage. PMCID:    Pubmed No.:26980009 Oroxylin A induces autophagy in human malignant glioma cells via the mTOR‐STAT3‐Notch signaling pathway PMCID:    Pubmed No.:25213258 Effects of gastrokine‑2 expression on gastric cancer cell apoptosis by activation of extrinsic apoptotic pathways PMCID:    Pubmed No.:25270871 Involvement of miR-143 in cisplatin resistance of gastric cancer cells via targeting IGF1R and BCL2 PMCID:    Pubmed No.:25492481 MicroRNA-181 functions as a tumor suppressor in non-small cell lung cancer (NSCLC) by targeting Bcl-2 PMCID:    Pubmed No.:25524579 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:None Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO 2-induced senescent mice PMCID:    Pubmed No.:27064004 PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells PMCID:    Pubmed No.:26860588 Blocking B7-1/CD28 Pathway Diminished Long-Range Brain Damage by Regulating the Immune and Inflammatory Responses in a Mouse Model of Intracerebral Hemorrhage. PMCID:    Pubmed No.:26980009 MicroRNA-30b functions as a tumour suppressor in human colorectal cancer by targeting KRAS, PIK3CD and BCL2 PMCID:    Pubmed No.:24293274 Hypoxia-Inducible Factor-1α Dependent Pathways Mediate the Renoprotective Role of Acetazolamide Against Renal Ischemia-Reperfusion Injury PMCID:    Pubmed No.:24335166 Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats PMCID:    Pubmed No.:24613208 Overexpression of BAT3 Alleviates Prion Protein Fragment PrP106‐126‐Induced Neuronal Apoptosis PMCID:    Pubmed No.:24629137 ZnO nanoparticle-induced oxidative stress triggers apoptosis by activating JNK signaling pathway in cultured primary astrocytes PMCID:    Pubmed No.:24624962 Exogenous High Mobility Group Box 1 Inhibits Apoptosis and Promotes the Proliferation of Lewis Cells via RAGE/TLR4‐Dependent Signal Pathways PMCID:    Pubmed No.:24673192 Racemic oleracein E increases the survival rate and attenuates memory impairment in D-galactose/NaNO2-induced senescent mice PMCID:    Pubmed No.:27064004 Phenolic alkaloid oleracein E attenuates oxidative stress and neurotoxicity in AlCl3-treated mice PMCID:    Pubmed No.:29054451 The regulator of calcineurin 1 increases adenine nucleotide translocator 1 and leads to mitochondrial dysfunctions PMCID:    Pubmed No.:27861892 LW‐213 induces G2/M cell cycle arrest through AKT/GSK3β/β‐catenin signaling pathway in human breast cancer cells PMCID:    Pubmed No.:25945460 Activation of cellular apoptosis in the caecal epithelium is associated with increased oxidative reactions in lactating goats after feeding a high-concentrate diet PMCID:    Pubmed No.:25590230 Clostridium butyricum exerts a neuroprotective effect in a mouse model of traumatic brain injury via the gut‐brain axis PMCID:    Pubmed No.:29193450 Downregulation of Foxc2 enhances apoptosis induced by 5-fluorouracil through activation of MAPK and AKT pathways in colorectal cancer PMCID:    Pubmed 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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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