Product Name :
IRAK1 (T381) polyclonal antibody Background :
Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IRAK1 (T381) polyclonal antibody detects endogenous levels of IRAK1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1. Conjugate :
Unconjugated Modification :
Unmodification
IRAK1 (T381) polyclonal antibody Background :
Three structurally related ligands for IL-1Rs have been described. These include two agonists, IL-1α and IL-1β, and a specific receptor antagonist, IL-1Rα. Two distinct receptors designated IL-1RI and IL-1RII have been identified, each of which belong to the Ig superfamily. The preponderance of evidence suggests IL-1RI to be the functional IL-1 receptor. Binding of IL-1 to its cognate receptor results in the activation of the NFκB signaling pathway. The IL-1-dependent kinase termed IRAK (for IL-1 receptor-associated kinase) co-immunoprecipitates with activated IL-1RI and has been implicated as an upstream mediator of NFκB activation. Additional support for this assertion comes from the fact that a related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFκB. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
IRAK1 (T381) polyclonal antibody detects endogenous levels of IRAK1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 350-400 of Human IRAK1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of IRAK1 (T381) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:THP-1 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug)
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.