PKAα/β cat (V191) polyclonal antibody 
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Product Name :
PKAα/β cat (V191) polyclonal antibody Background :
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PKAα/β cat (V191) polyclonal antibody detects endogenous levels of PKAα/β protein. Immunogen :
Synthetic peptide, corresponding to amino acids 160-210 of Human PKAα. Conjugate :
Unconjugated Modification :
Unmodification
PKAα/β cat (V191) polyclonal antibody Background :
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PKAα/β cat (V191) polyclonal antibody detects endogenous levels of PKAα/β protein. Immunogen :
Synthetic peptide, corresponding to amino acids 160-210 of Human PKAα. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PKAα/β cat (V191) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:The Testis tissue lysate of Rat(40ug) Lane4:The Testis tissue lysate of Mouse(40ug) -
Immunohistochemistry (IHC) analyzes of PKAα/β cat (V191) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
PMCID: Pubmed No.:24557578
Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
PMCID: Pubmed No.:25246240
Curcumin protects neurons from glutamate-induced excitotoxicity by membrane anchored AKAP79-PKA interaction network
PMCID: Pubmed No.:26170881
Curcumin regulates cell fate and metabolism by inhibiting hedgehog signaling in hepatic stellate cells
PMCID: Pubmed No.:25938627
Ruscogenin suppresses mouse neutrophil activation: Involvement of protein kinase A pathway
PMCID: Pubmed No.:26134424
Diosgenin inhibits superoxide generation in FMLP-activated mouse neutrophils via multiple pathways
PMCID: Pubmed No.:25246240
The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC
PMCID: Pubmed No.:24557578
Adipokine zinc-α2-glycoprotein alleviates lipopolysaccharide-induced inflammatory responses through the β3-AR/PKA/CREB pathway
PMCID: Pubmed No.:31260855
Zinc-α2-glycoprotein promotes skeletal muscle lipid metabolism in cold-stressed mice
PMCID: Pubmed No.:32863292
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.