Product Name :
PPAR-γ (I106) polyclonal antibody Background :
PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PPAR-γ (I106) polyclonal antibody detects endogenous levels of PPAR-γ protein . Immunogen :
Synthetic peptide, corresponding to amino acids 90-120 of Human PPAR-γ. Conjugate :
Unconjugated Modification :
Unmodification
PPAR-γ (I106) polyclonal antibody Background :
PPAR gamma is implicated in numerous diseases including obesity, diabetes, atherosclerosis and cancer. PPAR gamma activators include prostanoids, fatty acids, thiazolidinediones and N-(2-benzoylphenyl) tyrosine analogues. A key component in adipocyte differentiation and fat-specific gene expression, PPAR gamma may modulate macrophage functions such as proinflammatory activities, and stimulate oxidized low-density lipoprotein (x-LDL) uptake. A Pro12Ala polymorphism of the PPAR gamma2 gene has been reported to reduce transactivation activity in vitro. This substitution may affect the immune response to ox-LDL and be associated with type 2 diabetes. In addition, the Pro12Ala variant of the PPAR gamma2 gene maybe correlated with abdominal obesity in type 2 diabetes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
PPAR-γ (I106) polyclonal antibody detects endogenous levels of PPAR-γ protein . Immunogen :
Synthetic peptide, corresponding to amino acids 90-120 of Human PPAR-γ. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of PPAR-γ (I106) polyclonal antibody at 1:500 dilution Lane1:MEF whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of PPAR-γ (I106) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.
Cerebroprotective effects of ibuprofen on diabetic encephalopathy in rats
PMCID: Pubmed No.:24291733
PDGFR-β (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo
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Mark4 promotes adipogenesis and triggers apoptosis in 3T3-L1 adipocytes by activating JNK1 and inhibiting p38MAPK pathways.
PMCID: Pubmed No.:24989893
MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264. 7 macrophages
PMCID: Pubmed No.:26800097
Ibuprofen attenuates nephropathy in streptozotocin‑induced diabetic rats
PMCID: Pubmed No.:27109015
MiR-146a modulates macrophage polarization by inhibiting Notch1 pathway in RAW264. 7 macrophages
PMCID: Pubmed No.:26800097
Cerebroprotective effects of ibuprofen on diabetic encephalopathy in rats
PMCID: Pubmed No.:24291733
Pomegranate peel polyphenols inhibit lipid accumulation and enhance cholesterol efflux in raw264.7 macrophages
PMCID: Pubmed No.:27334099
The adipogenesis in infantile hemangioma and the expression of adipogenic-related genes
PMCID: Pubmed No.:31966516
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.