Cofilin1/2 (T88) polyclonal antibody 
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MSDS
SHIP
Product Name :
Cofilin1/2 (T88) polyclonal antibody Background :
Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin,thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan.Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cofilin1/2 (T88) polyclonal antibody detects endogenous levels of Cofilin1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 55-100 of Human Cofilin1. Conjugate :
Unconjugated Modification :
Unmodification
Cofilin1/2 (T88) polyclonal antibody Background :
Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin,thereby regulating the rapid cycling of Actin assembly and disassembly essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally associated Actin subunits changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors such as opsonized zymosan.Lim kinase 1, a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerise F-Actin. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cofilin1/2 (T88) polyclonal antibody detects endogenous levels of Cofilin1/2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 55-100 of Human Cofilin1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Cofilin1/2 (T88) polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:Mouse heart tissue lysate Lane3:Rat heart tissue lysate
WDR1 Promotes Cell Growth and Migration and Contributes to Malignant Phenotypes of Non-small Cell Lung Cancer through ADF/cofilin-mediated Actin Dynamics
PMCID: Pubmed No.:29989053
Knockout of Wdr1 results in cardiac hypertrophy and impaired cardiac function in adult mouse heart
PMCID: Pubmed No.:30794912
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.