Cdk2 (Q265) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
Cdk2 (Q265) polyclonal antibody Background :
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cdk2 (Q265) polyclonal antibody detects endogenous levels of Cdk2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 230-278 of Human Cdk2. Conjugate :
Unconjugated Modification :
Unmodification
Cdk2 (Q265) polyclonal antibody Background :
Cdk2 is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cdk2 (Q265) polyclonal antibody detects endogenous levels of Cdk2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 230-278 of Human Cdk2. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of Cdk2 (Q265) pAb at 1:500 dilution Lane1:K562 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:MEF whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of Cdk2 (Q265) pAb in paraffin-embedded human tonsil cancer tissue at 1:50.
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
Silencing DACH1 Promotes Esophageal Cancer Growth by Inhibiting TGF-β Signaling
PMCID: Pubmed No.:24743895
Selective ligands of estrogen receptor β discovered using pharmacophore mapping and structure-based virtual screening
PMCID: Pubmed No.:25176400
TPX2 Level Correlates with Hepatocellular Carcinoma Cell Proliferation, Apoptosis, and EMT
PMCID: Pubmed No.:26025609
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Selective ligands of estrogen receptor β discovered using pharmacophore mapping and structure-based virtual screening
PMCID: Pubmed No.:25176400
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
Silencing DACH1 Promotes Esophageal Cancer Growth by Inhibiting TGF-β Signaling
PMCID: Pubmed No.:24743895
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.