Product Name :
MEK-4 (K350) polyclonal antibody Background :
The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38β. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Western blot (WB) analysis of MEK-4 (K350) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug) Immunogen :
Synthetic peptide, corresponding to amino acids 330-380 of Human MEK-4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of MEK-4 (K350) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(20ug) Lane4:The Brain tissue lysate of Rat(40ug)
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  Immunohistochemistry (IHC) analyzes of MEK-4/ MAP2K4 (K350) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of MEK-4/ MAP2K4 (K350) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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