p53 (R375) polyclonal antibody 
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Product Name :
p53 (R375) polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 (R375) polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 341-390 of Human p53. Conjugate :
Unconjugated Modification :
Unmodification
p53 (R375) polyclonal antibody Background :
p53, a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosisrelated genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active (DNA-binding) conformations and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
p53 (R375) polyclonal antibody detects endogenous levels of p53 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 341-390 of Human p53. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of p53 (R375) polyclonal antibody -
Immunohistochemistry (IHC) analyzes of p53 (R375) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
p53-insensitive PUMA down-regulation is essential in the early phase of liver regeneration after partial hepatectomy in mice
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D-peptide inhibitors of the p53–MDM2 interaction for targeted molecular therapy of malignant neoplasms
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The toxicity evaluation of nano-trititanate with bactericidal properties in vitro
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TRAIL and doxorubicin combination enhances anti-glioblastoma effect based on passive tumor targeting of liposomes
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Akebia saponin D attenuates ibotenic acid-induced cognitive deficits and pro-apoptotic response in rats: involvement of MAPK signal pathway.
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Qi-Dan Fang ameliorates adriamycin-induced nephrotic syndrome rat model by enhancing renal function and inhibiting podocyte injury
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Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells
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TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway
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Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
Qi-Dan Fang ameliorates adriamycin-induced nephrotic syndrome rat model by enhancing renal function and inhibiting podocyte injury
PMCID: Pubmed No.:24389029
Molecular Mechanisms of Exopolysaccharide from Aphanothece halaphytica (EPSAH) Induced Apoptosis in HeLa Cells
PMCID: Pubmed No.:24466342
TRIM24 promotes glioma progression and enhances chemoresistance through activation of the PI3K/Akt signaling pathway
PMCID: Pubmed No.:24469053
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system
PMCID: Pubmed No.:32791387
Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure
PMCID: Pubmed No.:29725001
ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway
PMCID: Pubmed No.:33598419
Therapeutic propensity of ginsenosides Rg1 and Rg3 in rhabdomyolysis-induced acute kidney injury and renohepatic crosstalk in rats
PMCID: Pubmed No.:36580761
CopA3 peptide inhibits MDM2-p53 complex stability in colorectal cancers and activates p53 mediated cell death machinery
PMCID: Pubmed No.:36758667
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.