Product Name :
FAK (I855) polyclonal antibody Background :
Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FAK (I855) polyclonal antibody detects endogenous levels of FAK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 830-880 of Human FAK. Conjugate :
Unconjugated Modification :
Unmodification
FAK (I855) polyclonal antibody Background :
Focal adhesion kinase was initially identified as a major 125 kDa substrate for the intrinsic protein tyrosine kinase activity of Src-encoded pp60. The deduced amino acid sequence of FAK p125 has shown it to be a cytoplasmic protein tyrosine kinase whose sequence and structural organization are unique as compared to other proteins described to date. Localization of p125 by immunofluorescence suggests that it is primarily found in cellular focal adhesions leading to its designation as focal adhesion kinase (FAK). FAK is concentrated at the basal edge of only basal keratinocytes that are actively migrating and rapidly proliferating in repairing burn wounds, and is activated and localized to the focal adhesions of spreading keratinocytes in culture. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FAK (I855) polyclonal antibody detects endogenous levels of FAK protein. Immunogen :
Synthetic peptide, corresponding to amino acids 830-880 of Human FAK. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of FAK (I855) pAb at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug)
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Immunohistochemistry (IHC) analyzes of FAK (I855) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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Immunohistochemistry (IHC) analyzes of FAK (I855) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
PTK2-mediated degradation of ATG3 impedes cancer cells susceptible to DNA damage treatment
PMCID: Pubmed No.:28103122
Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro.
PMCID: Pubmed No.:24565841
Activation of matrix metalloproteinase-26 by HOXA10 promotes embryo adhesion in vitro.
PMCID: Pubmed No.:24565841
ADAM23 in Cardiomyocyte Inhibits Cardiac Hypertrophy by Targeting FAK‐AKT Signaling
PMCID: Pubmed No.:30371220
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.