Product Name :
c-Src (T523) polyclonal antibody Background :
The major translational products of each of the eight members of the Src gene family identified to date are membrane-associated tyrosine protein kinases that lack transmembrane and external amino acid sequences, thereby distinguishing this group from the receptor class of tyrosine kinases. Members of this group include c-Src, c-Yes, Fyn, Lck, Lyn, Blk and c-Fgr. The major Src gene encoded protein, c-Src (also designated pp60Src, Src p60 and proto-oncogene tyrosine protein kinase Src) is expressed in a broad range of tissue and cell types, although the highest levels of c-Src are detected in neuronal tissues and platelets. c-Src may play a role in events associated with both neuronal differentiation and maintenance of mature neuronal cell functions._x000D_ Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
c-Src (T523) polyclonal antibody detects endogenous levels of c-Src protein. Immunogen :
Synthetic peptide, corresponding to amino acids 480-530 of Human c-Src. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of c-Src (T523) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:U251 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Mouse(40ug) Lane4:The Brain tissue lysate of Rat(40ug)
• 
  Immunohistochemistry (IHC) analyzes of c-Src (T523) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---